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60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle

Author

Listed:
  • Christoph Leidig

    (Gene Center and Center of Integrated Protein Science Munich (CiPS-M), University of Munich)

  • Matthias Thoms

    (Heidelberg University Biochemistry Center (BZH))

  • Iris Holdermann

    (Heidelberg University Biochemistry Center (BZH))

  • Bettina Bradatsch

    (Heidelberg University Biochemistry Center (BZH))

  • Otto Berninghausen

    (Gene Center and Center of Integrated Protein Science Munich (CiPS-M), University of Munich)

  • Gert Bange

    (Heidelberg University Biochemistry Center (BZH)
    Present address: LOEWE Center for Synthetic Microbiology (Synmikro), Marburg, Germany)

  • Irmgard Sinning

    (Heidelberg University Biochemistry Center (BZH))

  • Ed Hurt

    (Heidelberg University Biochemistry Center (BZH))

  • Roland Beckmann

    (Gene Center and Center of Integrated Protein Science Munich (CiPS-M), University of Munich)

Abstract

During eukaryotic ribosome biogenesis, nascent ribosomal RNA (rRNA) forms pre-ribosomal particles containing ribosomal proteins and assembly factors. Subsequently, these immature rRNAs are processed and remodelled. Little is known about the premature assembly states of rRNAs and their structural rearrangement during ribosome biogenesis. Using cryo-EM we characterize a pre-60S particle, where the 5S rRNA and its associated ribosomal proteins L18 and L5 (5S ribonucleoprotein (RNP)) are rotated by almost 180° when compared with the mature subunit. Consequently, neighbouring 25S rRNA helices that protrude from the base of the central protuberance are deformed. This altered topology is stabilized by nearby assembly factors (Rsa4 and Nog1), which were identified by fitting their three-dimensional structures into the cryo-EM density. We suggest that the 5S RNP performs a semicircular movement during 60S biogenesis to adopt its final position, fulfilling a chaperone-like function in guiding the flanking 25S rRNA helices of the central protuberance to their final topology.

Suggested Citation

  • Christoph Leidig & Matthias Thoms & Iris Holdermann & Bettina Bradatsch & Otto Berninghausen & Gert Bange & Irmgard Sinning & Ed Hurt & Roland Beckmann, 2014. "60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle," Nature Communications, Nature, vol. 5(1), pages 1-8, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4491
    DOI: 10.1038/ncomms4491
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    Cited by:

    1. Ana Martinez-Val & Dorte B. Bekker-Jensen & Sophia Steigerwald & Claire Koenig & Ole Østergaard & Adi Mehta & Trung Tran & Krzysztof Sikorski & Estefanía Torres-Vega & Ewa Kwasniewicz & Sólveig Hlín B, 2021. "Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution," Nature Communications, Nature, vol. 12(1), pages 1-17, December.

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