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Imaging live cell in micro-liquid enclosure by X-ray laser diffraction

Author

Listed:
  • Takashi Kimura

    (Research Institute for Electronic Science, Hokkaido University)

  • Yasumasa Joti

    (Japan Synchrotron Radiation Research Institute/SPring-8)

  • Akemi Shibuya

    (RIKEN SPring-8 Center)

  • Changyong Song

    (RIKEN SPring-8 Center)

  • Sangsoo Kim

    (RIKEN SPring-8 Center)

  • Kensuke Tono

    (Japan Synchrotron Radiation Research Institute/SPring-8)

  • Makina Yabashi

    (RIKEN SPring-8 Center)

  • Masatada Tamakoshi

    (Tokyo University of Pharmacy and Life Sciences)

  • Toshiyuki Moriya

    (Institute of Environmental Microbiology, Kyowa-kako Co. Ltd.)

  • Tairo Oshima

    (Institute of Environmental Microbiology, Kyowa-kako Co. Ltd.)

  • Tetsuya Ishikawa

    (RIKEN SPring-8 Center)

  • Yoshitaka Bessho

    (RIKEN SPring-8 Center)

  • Yoshinori Nishino

    (Research Institute for Electronic Science, Hokkaido University)

Abstract

Emerging X-ray free-electron lasers with femtosecond pulse duration enable single-shot snapshot imaging almost free from sample damage by outrunning major radiation damage processes. In bioimaging, it is essential to keep the sample close to its natural state. Conventional high-resolution imaging, however, suffers from severe radiation damage that hinders live cell imaging. Here we present a method for capturing snapshots of live cells kept in a micro-liquid enclosure array by X-ray laser diffraction. We place living Microbacterium lacticum cells in an enclosure array and successively expose each enclosure to a single X-ray laser pulse from the SPring-8 Angstrom Compact Free-Electron Laser. The enclosure itself works as a guard slit and allows us to record a coherent diffraction pattern from a weakly-scattering submicrometre-sized cell with a clear fringe extending up to a 28-nm full-period resolution. The reconstructed image reveals living whole-cell structures without any staining, which helps advance understanding of intracellular phenomena.

Suggested Citation

  • Takashi Kimura & Yasumasa Joti & Akemi Shibuya & Changyong Song & Sangsoo Kim & Kensuke Tono & Makina Yabashi & Masatada Tamakoshi & Toshiyuki Moriya & Tairo Oshima & Tetsuya Ishikawa & Yoshitaka Bess, 2014. "Imaging live cell in micro-liquid enclosure by X-ray laser diffraction," Nature Communications, Nature, vol. 5(1), pages 1-7, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4052
    DOI: 10.1038/ncomms4052
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    Cited by:

    1. Hirokatsu Yumoto & Takahisa Koyama & Akihiro Suzuki & Yasumasa Joti & Yoshiya Niida & Kensuke Tono & Yoshitaka Bessho & Makina Yabashi & Yoshinori Nishino & Haruhiko Ohashi, 2022. "High-fluence and high-gain multilayer focusing optics to enhance spatial resolution in femtosecond X-ray laser imaging," Nature Communications, Nature, vol. 13(1), pages 1-8, December.

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