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Highly efficient methane biocatalysis revealed in a methanotrophic bacterium

Author

Listed:
  • M. G. Kalyuzhnaya

    (University of Washington)

  • S. Yang

    (University of Washington
    Present addresses: Shandong Province Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, Changcheng Road 700, Chengyang District, Qingdao 266109, China, and Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China)

  • O. N. Rozova

    (G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences)

  • N. E. Smalley

    (University of Washington)

  • J. Clubb

    (University of Washington)

  • A. Lamb

    (University of Washington)

  • G. A. Nagana Gowda

    (Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington)

  • D. Raftery

    (Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington)

  • Y. Fu

    (University of Washington)

  • F. Bringel

    (Equipe Adaptations et Interactions Microbiennes dans l’Environnement, UMR 7156 UdS – CNRS Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg)

  • S. Vuilleumier

    (Equipe Adaptations et Interactions Microbiennes dans l’Environnement, UMR 7156 UdS – CNRS Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg)

  • D. A. C. Beck

    (University of Washington
    eScience Institute, University of Washington)

  • Y. A. Trotsenko

    (G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences)

  • V. N. Khmelenina

    (G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences)

  • M. E. Lidstrom

    (University of Washington
    University of Washington)

Abstract

Methane is an essential component of the global carbon cycle and one of the most powerful greenhouse gases, yet it is also a promising alternative source of carbon for the biological production of value-added chemicals. Aerobic methane-consuming bacteria (methanotrophs) represent a potential biological platform for methane-based biocatalysis. Here we use a multi-pronged systems-level approach to reassess the metabolic functions for methane utilization in a promising bacterial biocatalyst. We demonstrate that methane assimilation is coupled with a highly efficient pyrophosphate-mediated glycolytic pathway, which under oxygen limitation participates in a novel form of fermentation-based methanotrophy. This surprising discovery suggests a novel mode of methane utilization in oxygen-limited environments, and opens new opportunities for a modular approach towards producing a variety of excreted chemical products using methane as a feedstock.

Suggested Citation

  • M. G. Kalyuzhnaya & S. Yang & O. N. Rozova & N. E. Smalley & J. Clubb & A. Lamb & G. A. Nagana Gowda & D. Raftery & Y. Fu & F. Bringel & S. Vuilleumier & D. A. C. Beck & Y. A. Trotsenko & V. N. Khmele, 2013. "Highly efficient methane biocatalysis revealed in a methanotrophic bacterium," Nature Communications, Nature, vol. 4(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3785
    DOI: 10.1038/ncomms3785
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    Cited by:

    1. Simone Bachleitner & Özge Ata & Diethard Mattanovich, 2023. "The potential of CO2-based production cycles in biotechnology to fight the climate crisis," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    2. Yeonhwa Yu & Yongfan Shi & Young Wan Kwon & Yoobin Choi & Yusik Kim & Jeong-Geol Na & June Huh & Jeewon Lee, 2024. "A rationally designed miniature of soluble methane monooxygenase enables rapid and high-yield methanol production in Escherichia coli," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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