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Localized cell stimulation by nitric oxide using a photoactive porous coordination polymer platform

Author

Listed:
  • Stéphane Diring

    (Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University)

  • Dan Ohtan Wang

    (Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University)

  • Chiwon Kim

    (Graduate School of Engineering, Kyoto University)

  • Mio Kondo

    (Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University)

  • Yong Chen

    (Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University)

  • Susumu Kitagawa

    (Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University
    Graduate School of Engineering, Kyoto University)

  • Ken-ichiro Kamei

    (Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University)

  • Shuhei Furukawa

    (Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University)

Abstract

Functional cellular substrates for localized cell stimulation by small molecules provide an opportunity to control and monitor cell signalling networks chemically in time and space. However, despite improvements in the controlled delivery of bioactive compounds, the precise localization of gaseous biomolecules at the single-cell level remains challenging. Here we target nitric oxide, a crucial signalling molecule with site-specific and concentration-dependent activities, and we report a synthetic strategy for developing spatiotemporally controllable nitric oxide-releasing platforms based on photoactive porous coordination polymers. By organizing molecules with poor reactivity into polymer structures, we observe increased photoreactivity and adjustable release using light irradiation. We embed photoactive polymer crystals in a biocompatible matrix and achieve precisely controlled nitric oxide delivery at the cellular level via localized two-photon laser activation. The biological relevance of the exogenous nitric oxide produced by this strategy is evidenced by an intracellular change in calcium concentration, mediated by nitric oxide-responsive plasma membrane channel proteins.

Suggested Citation

  • Stéphane Diring & Dan Ohtan Wang & Chiwon Kim & Mio Kondo & Yong Chen & Susumu Kitagawa & Ken-ichiro Kamei & Shuhei Furukawa, 2013. "Localized cell stimulation by nitric oxide using a photoactive porous coordination polymer platform," Nature Communications, Nature, vol. 4(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3684
    DOI: 10.1038/ncomms3684
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    Cited by:

    1. Meizhen Gao & Rui-Kang Huang & Bin Zheng & Pengfei Wang & Qi Shi & Wei-Xiong Zhang & Jinxiang Dong, 2022. "Large breathing effect in ZIF-65(Zn) with expansion and contraction of the SOD cage," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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