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Real-time in vivo imaging of invasive- and biomaterial-associated bacterial infections using fluorescently labelled vancomycin

Author

Listed:
  • Marleen van Oosten

    (University of Groningen, University Medical Center Groningen (UMCG)
    BioOptical Imaging Center, University of Groningen, UMCG)

  • Tina Schäfer

    (Institut für Molekulare Infektionsbiologie, Universität Würzburg)

  • Joost A. C. Gazendam

    (University of Groningen, University Medical Center Groningen (UMCG)
    Medical Center Alkmaar)

  • Knut Ohlsen

    (Institut für Molekulare Infektionsbiologie, Universität Würzburg)

  • Eleni Tsompanidou

    (University of Groningen, University Medical Center Groningen (UMCG))

  • Marcus C. de Goffau

    (University of Groningen, University Medical Center Groningen (UMCG))

  • Hermie J. M. Harmsen

    (University of Groningen, University Medical Center Groningen (UMCG))

  • Lucia M. A. Crane

    (BioOptical Imaging Center, University of Groningen, UMCG)

  • Ed Lim

    (Caliper—a PerkinElmer Company)

  • Kevin P. Francis

    (Caliper—a PerkinElmer Company)

  • Lael Cheung

    (LI-COR Biosciences)

  • Michael Olive

    (LI-COR Biosciences)

  • Vasilis Ntziachristos

    (Technische Universität München & Helmholtz Zentrum)

  • Jan Maarten van Dijl

    (University of Groningen, University Medical Center Groningen (UMCG))

  • Gooitzen M. van Dam

    (BioOptical Imaging Center, University of Groningen, UMCG)

Abstract

Invasive and biomaterial-associated infections in humans are often difficult to diagnose and treat. Here, guided by recent advances in clinically relevant optical imaging technologies, we explore the use of fluorescently labelled vancomycin (vanco-800CW) to specifically target and detect infections caused by Gram-positive bacteria. The application potential of vanco-800CW for real-time in vivo imaging of bacterial infections is assessed in a mouse myositis model and a human post-mortem implant model. We show that vanco-800CW can specifically detect Gram-positive bacterial infections in our mouse myositis model, discriminate bacterial infections from sterile inflammation in vivo and detect biomaterial-associated infections in the lower leg of a human cadaver. We conclude that vanco-800CW has a high potential for enhanced non-invasive diagnosis of infections with Gram-positive bacteria and is a promising candidate for early-phase clinical trials.

Suggested Citation

  • Marleen van Oosten & Tina Schäfer & Joost A. C. Gazendam & Knut Ohlsen & Eleni Tsompanidou & Marcus C. de Goffau & Hermie J. M. Harmsen & Lucia M. A. Crane & Ed Lim & Kevin P. Francis & Lael Cheung & , 2013. "Real-time in vivo imaging of invasive- and biomaterial-associated bacterial infections using fluorescently labelled vancomycin," Nature Communications, Nature, vol. 4(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3584
    DOI: 10.1038/ncomms3584
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    Cited by:

    1. Yunmin Yang & Binbin Chu & Jiayi Cheng & Jiali Tang & Bin Song & Houyu Wang & Yao He, 2022. "Bacteria eat nanoprobes for aggregation-enhanced imaging and killing diverse microorganisms," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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