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Haemogenic endocardium contributes to transient definitive haematopoiesis

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  • Haruko Nakano

    (University of California, Los Angeles
    Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles)

  • Xiaoqian Liu

    (University of California, Los Angeles)

  • Armin Arshi

    (University of California, Los Angeles)

  • Yasuhiro Nakashima

    (University of California, Los Angeles)

  • Ben van Handel

    (University of California, Los Angeles)

  • Rajkumar Sasidharan

    (University of California, Los Angeles)

  • Andrew W. Harmon

    (University of California, Los Angeles
    Molecular Biology Institute, University of California, Los Angeles)

  • Jae-Ho Shin

    (University of California, Los Angeles)

  • Robert J. Schwartz

    (University of Houston)

  • Simon J. Conway

    (Medical and Molecular Genetics, Biochemistry and Molecular Biology, Indiana University School of Medicine
    Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indiana University)

  • Richard P. Harvey

    (the Victor Chang Cardiac Research Institute
    St Vincent’s Clinical School, University of New South Wales, Darlinghurst)

  • Mohammad Pashmforoush

    (Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of Southern California Keck School of Medicine)

  • Hanna K. A. Mikkola

    (University of California, Los Angeles
    Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles
    Molecular Biology Institute, University of California, Los Angeles
    Jonsson Comprehensive Cancer Center, University of California, Los Angeles)

  • Atsushi Nakano

    (University of California, Los Angeles
    Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles
    Molecular Biology Institute, University of California, Los Angeles
    Jonsson Comprehensive Cancer Center, University of California, Los Angeles)

Abstract

Haematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial and haematopoietic lineages, it remains unknown whether the mammalian heart tube serves as a haemogenic organ akin to the dorsal aorta. Here we examine the haemogenic activity of the developing endocardium. Mouse heart explants generate myeloid and erythroid colonies in the absence of circulation. Haemogenic activity arises from a subset of endocardial cells in the outflow cushion and atria earlier than in the aorta-gonad-mesonephros region, and is transient and definitive in nature. Interestingly, key cardiac transcription factors, Nkx2–5 and Isl1, are expressed in and required for the haemogenic population of the endocardium. Together, these data suggest that a subset of endocardial/endothelial cells serve as a de novo source for transient definitive haematopoietic progenitors.

Suggested Citation

  • Haruko Nakano & Xiaoqian Liu & Armin Arshi & Yasuhiro Nakashima & Ben van Handel & Rajkumar Sasidharan & Andrew W. Harmon & Jae-Ho Shin & Robert J. Schwartz & Simon J. Conway & Richard P. Harvey & Moh, 2013. "Haemogenic endocardium contributes to transient definitive haematopoiesis," Nature Communications, Nature, vol. 4(1), pages 1-10, June.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2569
    DOI: 10.1038/ncomms2569
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    Cited by:

    1. Norika Liu & Naofumi Kawahira & Yasuhiro Nakashima & Haruko Nakano & Akiyasu Iwase & Yasunobu Uchijima & Mei Wang & Sean M. Wu & Susumu Minamisawa & Hiroki Kurihara & Atsushi Nakano, 2023. "Notch and retinoic acid signals regulate macrophage formation from endocardium downstream of Nkx2-5," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Dorothee Bornhorst & Amulya V. Hejjaji & Lena Steuter & Nicole M. Woodhead & Paul Maier & Alessandra Gentile & Alice Alhajkadour & Octavia Santis Larrain & Michael Weber & Khrievono Kikhi & Stefan Gue, 2024. "The heart is a resident tissue for hematopoietic stem and progenitor cells in zebrafish," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    3. João Cardeira-da-Silva & Qianchen Wang & Pooja Sagvekar & Janita Mintcheva & Stephan Latting & Stefan Günther & Radhan Ramadass & Michail Yekelchyk & Jens Preussner & Mario Looso & Jan Philipp Junker , 2024. "Antigen presentation plays positive roles in the regenerative response to cardiac injury in zebrafish," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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