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Switching of myosin-V motion between the lever-arm swing and Brownian search-and-catch

Author

Listed:
  • Keisuke Fujita

    (Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University)

  • Mitsuhiro Iwaki

    (Graduate School of Medicine, Osaka University
    Laboratory for Cell Dynamics Observation, Quantitative Biology Center)

  • Atsuko H. Iwane

    (Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University)

  • Lorenzo Marcucci

    (Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University)

  • Toshio Yanagida

    (Soft Biosystem Group, Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University
    Laboratory for Cell Dynamics Observation, Quantitative Biology Center)

Abstract

Motor proteins are force-generating nanomachines that are highly adaptable to their ever-changing biological environments and have a high energy conversion efficiency. Here we constructed an imaging system that uses optical tweezers and a DNA handle to visualize elementary mechanical processes of a nanomachine under load. We apply our system to myosin-V, a well-known motor protein that takes 72 nm 'hand-over-hand' steps composed of a 'lever-arm swing' and a 'Brownian search-and-catch'. We find that the lever-arm swing generates a large proportion of the force at low load (

Suggested Citation

  • Keisuke Fujita & Mitsuhiro Iwaki & Atsuko H. Iwane & Lorenzo Marcucci & Toshio Yanagida, 2012. "Switching of myosin-V motion between the lever-arm swing and Brownian search-and-catch," Nature Communications, Nature, vol. 3(1), pages 1-9, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1934
    DOI: 10.1038/ncomms1934
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    Cited by:

    1. Qing-Miao Nie & Akio Togashi & Takeshi N Sasaki & Mitsunori Takano & Masaki Sasai & Tomoki P Terada, 2014. "Coupling of Lever Arm Swing and Biased Brownian Motion in Actomyosin," PLOS Computational Biology, Public Library of Science, vol. 10(4), pages 1-13, April.

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