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Skeletal muscle stem cells adopt a dormant cell state post mortem and retain regenerative capacity

Author

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  • Mathilde Latil

    (Human Histopathology and Animal Models, Infection & Epidemiology Departement, Institut Pasteur
    Faculty of Medicine, Université Versailles Saint Quentin en Yvelines UVSQ
    Université Paris Est)

  • Pierre Rocheteau

    (Stem Cells & Development, CNRS URA 2578, Institut Pasteur)

  • Laurent Châtre

    (Yeast Molecular genetics, Institut Pasteur, CNRS UMR 3525)

  • Serena Sanulli

    (Stem Cells & Development, CNRS URA 2578, Institut Pasteur
    Present address: Institut Curie, Mechanisms of repression by polycomb group proteins, Unit of genetics and developmental biology, INSERM U934, CNRS UMR3215, 26 rue d'Ulm, Paris, France.)

  • Sylvie Mémet

    (Molecular Mycology Unit, Infection & Epidemiology Departement, Institut Pasteur
    CNRS, URA3012)

  • Miria Ricchetti

    (Yeast Molecular genetics, Institut Pasteur, CNRS UMR 3525)

  • Shahragim Tajbakhsh

    (Stem Cells & Development, CNRS URA 2578, Institut Pasteur)

  • Fabrice Chrétien

    (Human Histopathology and Animal Models, Infection & Epidemiology Departement, Institut Pasteur
    Faculty of Medicine, Université Versailles Saint Quentin en Yvelines UVSQ
    Assistance Publique Hôpitaux de Paris, AP-HP, Service d'Anatomie Pathologique et de Médecine légale, Hôpital Raymond Poincaré)

Abstract

The accessibility to stem cells from healthy or diseased individuals, and the maintenance of their potency are challenging issues for stem cell biology. Here we report the isolation of viable and functional skeletal myogenic cells from humans up to 17 days, and mice up to 14 days post mortem, much longer beyond previous reports. Muscle stem cells are enriched in post mortem tissue, suggesting a selective survival advantage compared with other cell types. Transplantation of mouse muscle and haematopoietic stem cells regenerates tissues robustly. Cellular quiescence contributes to this cell viability where cells adopt a reversible dormant state characterized by reduced metabolic activity, a prolonged lag phase before the first cell division, elevated levels of reactive oxygen species and a transcriptional status less primed for commitment. Finally, severe hypoxia, or anoxia is critical for maintaining stem cell viability and regenerative capacity. Thus, these cells provide a useful resource for studying stem cell biology.

Suggested Citation

  • Mathilde Latil & Pierre Rocheteau & Laurent Châtre & Serena Sanulli & Sylvie Mémet & Miria Ricchetti & Shahragim Tajbakhsh & Fabrice Chrétien, 2012. "Skeletal muscle stem cells adopt a dormant cell state post mortem and retain regenerative capacity," Nature Communications, Nature, vol. 3(1), pages 1-12, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1890
    DOI: 10.1038/ncomms1890
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    Cited by:

    1. Mylène Fefeu & Michael Blatzer & Anita Kneppers & David Briand & Pierre Rocheteau & Alexandre Haroche & David Hardy & Mélanie Juchet-Martin & Anne Danckaert & François Coudoré & Abdulkarim Tutakhail &, 2024. "Serotonin reuptake inhibitors improve muscle stem cell function and muscle regeneration in male mice," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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