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Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana

Author

Listed:
  • Randor Radakovits

    (Colorado School of Mines)

  • Robert E. Jinkerson

    (Colorado School of Mines)

  • Susan I. Fuerstenberg

    (Genome Project Solutions)

  • Hongseok Tae

    (Data Analysis Core, Virginia Bioinformatics Institute, Virginia Tech)

  • Robert E. Settlage

    (Data Analysis Core, Virginia Bioinformatics Institute, Virginia Tech)

  • Jeffrey L. Boore

    (Genome Project Solutions
    University of California)

  • Matthew C. Posewitz

    (Colorado School of Mines)

Abstract

The potential use of algae in biofuels applications is receiving significant attention. However, none of the current algal model species are competitive production strains. Here we present a draft genome sequence and a genetic transformation method for the marine microalga Nannochloropsis gaditana CCMP526. We show that N. gaditana has highly favourable lipid yields, and is a promising production organism. The genome assembly includes nuclear (~29 Mb) and organellar genomes, and contains 9,052 gene models. We define the genes required for glycerolipid biogenesis and detail the differential regulation of genes during nitrogen-limited lipid biosynthesis. Phylogenomic analysis identifies genetic attributes of this organism, including unique stramenopile photosynthesis genes and gene expansions that may explain the distinguishing photoautotrophic phenotypes observed. The availability of a genome sequence and transformation methods will facilitate investigations into N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga.

Suggested Citation

  • Randor Radakovits & Robert E. Jinkerson & Susan I. Fuerstenberg & Hongseok Tae & Robert E. Settlage & Jeffrey L. Boore & Matthew C. Posewitz, 2012. "Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana," Nature Communications, Nature, vol. 3(1), pages 1-11, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1688
    DOI: 10.1038/ncomms1688
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    Cited by:

    1. Sun, Han & Wu, Tao & Chen, Stephenie Hiu Yuet & Ren, Yuanyuan & Yang, Shufang & Huang, Junchao & Mou, Haijin & Chen, Feng, 2021. "Powerful tools for productivity improvements in microalgal production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 152(C).
    2. Christopher W. Gee & Johan Andersen-Ranberg & Ethan Boynton & Rachel Z. Rosen & Danielle Jorgens & Patricia Grob & Hoi-Ying N. Holman & Krishna K. Niyogi, 2024. "Implicating the red body of Nannochloropsis in forming the recalcitrant cell wall polymer algaenan," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    3. Patel, Anil Kumar & Singhania, Reeta Rani & Dong, Cheng-Di & Obulisami, Parthiba Karthikeyan & Sim, Sang Jun, 2021. "Mixotrophic biorefinery: A promising algal platform for sustainable biofuels and high value coproducts," Renewable and Sustainable Energy Reviews, Elsevier, vol. 152(C).

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