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A plasmid-based multigene expression system for mammalian cells

Author

Listed:
  • Andrijana Kriz

    (Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology)

  • Katharina Schmid

    (Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology)

  • Nadia Baumgartner

    (Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology)

  • Urs Ziegler

    (Center for Microscopy and Image Analysis, University of Zurich)

  • Imre Berger

    (EMBL)

  • Kurt Ballmer-Hofer

    (Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology)

  • Philipp Berger

    (Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology)

Abstract

The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary experimental biological research. The coexpression of fluorescently tagged sensors is required to simultaneously analyse multiple parameters in living cells and the coexpression of several proteins is necessary to manipulate cell fate in stem cell biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. In this paper we describe MultiLabel, a novel and highly efficient modular plasmid-based eukaryotic expression system. Independent expression vectors are assembled by a Cre/LoxP reaction into a plasmid with multiple expression cassettes. MultiLabel enables rapid construction of multigene expression vectors for the single-step creation of transiently or stably transfected mammalian cells.

Suggested Citation

  • Andrijana Kriz & Katharina Schmid & Nadia Baumgartner & Urs Ziegler & Imre Berger & Kurt Ballmer-Hofer & Philipp Berger, 2010. "A plasmid-based multigene expression system for mammalian cells," Nature Communications, Nature, vol. 1(1), pages 1-6, December.
    • Handle: RePEc:nat:natcom:v:1:y:2010:i:1:d:10.1038_ncomms1120
    DOI: 10.1038/ncomms1120
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    Cited by:

    1. Baju Joy & Yubin Cai & David C. Bono & Deblina Sarkar, 2022. "Cell Rover—a miniaturized magnetostrictive antenna for wireless operation inside living cells," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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