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A role for TREX components in the release of spliced mRNA from nuclear speckle domains

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Listed:
  • Anusha P. Dias

    (Harvard Medical School)

  • Kobina Dufu

    (Harvard Medical School)

  • Haixin Lei

    (Harvard Medical School)

  • Robin Reed

    (Harvard Medical School)

Abstract

The TREX complex, which functions in mRNA export, is recruited to mRNA during splicing. Both the splicing machinery and the TREX complex are concentrated in 20–50 discrete foci known as nuclear speckle domains. In this study, we use a model system where DNA constructs are microinjected into HeLa cell nuclei, to follow the fates of the transcripts. We show that transcripts lacking functional splice sites, which are inefficiently exported, do not associate with nuclear speckle domains but are instead distributed throughout the nucleoplasm. In contrast, pre-mRNAs containing functional splice sites accumulate in nuclear speckles, and our data suggest that splicing occurs in these domains. When the TREX components UAP56 or Aly are knocked down, spliced mRNA, as well as total polyA+ RNA, accumulates in nuclear speckle domains. Together, our data raise the possibility that pre-mRNA undergoes splicing in nuclear speckle domains, before their release by TREX components for efficient export to the cytoplasm.

Suggested Citation

  • Anusha P. Dias & Kobina Dufu & Haixin Lei & Robin Reed, 2010. "A role for TREX components in the release of spliced mRNA from nuclear speckle domains," Nature Communications, Nature, vol. 1(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:1:y:2010:i:1:d:10.1038_ncomms1103
    DOI: 10.1038/ncomms1103
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    Cited by:

    1. Rudrarup Bhattacharjee & Lachlan A. Jolly & Mark A. Corbett & Ing Chee Wee & Sushma R. Rao & Alison E. Gardner & Tarin Ritchie & Eline J. H. Hugte & Ummi Ciptasari & Sandra Piltz & Jacqueline E. Noll , 2024. "Compromised transcription-mRNA export factor THOC2 causes R-loop accumulation, DNA damage and adverse neurodevelopment," Nature Communications, Nature, vol. 15(1), pages 1-25, December.
    2. Elizabeth A. Werren & Geneva R. LaForce & Anshika Srivastava & Delia R. Perillo & Shaokun Li & Katherine Johnson & Safa Baris & Brandon Berger & Samantha L. Regan & Christian D. Pfennig & Sonja Munnik, 2024. "TREX tetramer disruption alters RNA processing necessary for corticogenesis in THOC6 Intellectual Disability Syndrome," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

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