IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-57162-5.html
   My bibliography  Save this article

The Drosophila epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour

Author

Listed:
  • Vasiliki S. Lalioti

    (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid)

  • Ana-Citlali Gradilla

    (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid
    University of Exeter)

  • Carlos Jiménez-Jiménez

    (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid)

  • Clara Fernández-Pardo

    (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid)

  • David Sánchez-Hernández

    (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid)

  • Adrián Aguirre-Tamaral

    (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid
    University of Graz)

  • Irene Sánchez-Platero

    (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid)

  • Sheila Jordán-Àlvarez

    (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid)

  • James G. Wakefield

    (University of Exeter)

  • Isabel Guerrero

    (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid)

Abstract

During Drosophila epithelial development, dynamic signalling filopodia (cytonemes) establish direct contacts between distant cells to facilitate the formation of the Hedgehog signalling gradient. However, not much is known about how cytonemes are regulated. In this study, we show that cytoneme dynamics and Hedgehog signalling in the Drosophila epithelia depend on the Epidermal Growth Factor pathway and on its downstream effector Ras1. We describe that EGFR/Ras1 pathway is required to maintain in the wing disc epithelium the basal plasma membrane levels of Interference Hedgehog (Ihog), a critical Hh co-receptor and adhesion protein. In addition, our data demonstrate that filamin A or Cheerio in Drosophila, responds to both Ihog and EGFR pathway and recruited to the basal site of the plasma membrane. This recruitment contributes to Ihog’s role in stabilizing cytonemes.

Suggested Citation

  • Vasiliki S. Lalioti & Ana-Citlali Gradilla & Carlos Jiménez-Jiménez & Clara Fernández-Pardo & David Sánchez-Hernández & Adrián Aguirre-Tamaral & Irene Sánchez-Platero & Sheila Jordán-Àlvarez & James G, 2025. "The Drosophila epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57162-5
    DOI: 10.1038/s41467-025-57162-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-57162-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-57162-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Adrián Aguirre-Tamaral & Manuel Cambón & David Poyato & Juan Soler & Isabel Guerrero, 2022. "Predictive model for cytoneme guidance in Hedgehog signaling based on Ihog- Glypicans interaction," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Marcos Julián Cardozo & Luisa Sánchez-Arrones & África Sandonis & Cristina Sánchez-Camacho & Gaia Gestri & Stephen W. Wilson & Isabel Guerrero & Paola Bovolenta, 2014. "Cdon acts as a Hedgehog decoy receptor during proximal-distal patterning of the optic vesicle," Nature Communications, Nature, vol. 5(1), pages 1-13, September.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Blanco, Beatriz & Palma, Roberto & Hurtado, Manuel & Jiménez, Gema & Griñán-Lisón, Carmen & Melchor, Juan & Marchal, Juan Antonio & Gomez, Hector & Rus, Guillermo & Soler, Juan, 2025. "Modeling low-intensity ultrasound mechanotherapy impact on growing cancer stem cells," Mathematics and Computers in Simulation (MATCOM), Elsevier, vol. 228(C), pages 87-102.
    2. Beatriz Blanco & Juan Campos & Juan Melchor & Juan Soler, 2021. "Modeling Interactions among Migration, Growth and Pressure in Tumor Dynamics," Mathematics, MDPI, vol. 9(12), pages 1-19, June.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57162-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.