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Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels

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  • Saku T. Kijima

    (Nagoya University, Furo-cho, Chikusa-ku
    1-1-1 Higashi
    National Institute of Advanced Industrial Science and Technology (AIST))

  • Takema Sasaki

    (Nagoya University, Furo-cho, Chikusa-ku)

  • Yuichiro Kikushima

    (Nagoya University, Furo-cho, Chikusa-ku)

  • Daisuke Inoue

    (Kyusyu University)

  • Shingo Sakamoto

    (1-1-1 Higashi
    National Institute of Advanced Industrial Science and Technology (AIST))

  • Yuki Kondo

    (Osaka University)

  • Soichi Inagaki

    (The University of Tokyo)

  • Masatoshi Yamaguchi

    (Saitama University)

  • Nobutaka Mitsuda

    (1-1-1 Higashi
    National Institute of Advanced Industrial Science and Technology (AIST))

  • Yoshihisa Oda

    (Nagoya University, Furo-cho, Chikusa-ku)

Abstract

Cell wall patterning is central to determining the shape and function of plant cells. Protoxylem and metaxylem vessel cells deposit banded and pitted cell walls, respectively, which enable their distinctive water transport capabilities. Here, we show that the pitted cell wall pattern in metaxylem vessels is specified by transcriptional control of actin polymerization. A newly isolated allele of KNOTTED-LIKE HOMEOBOX TRANSCRIPTION FACTOR 7 (KNAT7) was associated with the formation of banded cell walls in metaxylem vessels. Loss of KNAT7 caused misexpression of FORMIN HOMOLOGY DOMAIN CONTAINING PROTEIN11 (FH11) in the metaxylem, which in turn caused rearrangements of ROP GTPases and microtubules in banded patterns. FH11 function required its plasma membrane anchoring and actin polymerization activity. These results suggest that excessive actin polymerization at the plasma membrane abolishes the pitted cell wall formation and promotes banded cell wall formation in metaxylem vessels. This study unveils the importance of proper control of actin polymerization for cell wall pattern determination.

Suggested Citation

  • Saku T. Kijima & Takema Sasaki & Yuichiro Kikushima & Daisuke Inoue & Shingo Sakamoto & Yuki Kondo & Soichi Inagaki & Masatoshi Yamaguchi & Nobutaka Mitsuda & Yoshihisa Oda, 2025. "Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-56866-y
    DOI: 10.1038/s41467-025-56866-y
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    References listed on IDEAS

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    1. René Schneider & Kris van’t Klooster & Kelsey L. Picard & Jasper Gucht & Taku Demura & Marcel Janson & Arun Sampathkumar & Eva E. Deinum & Tijs Ketelaar & Staffan Persson, 2021. "Long-term single-cell imaging and simulations of microtubules reveal principles behind wall patterning during proto-xylem development," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Yuki Sugiyama & Yoshinobu Nagashima & Mayumi Wakazaki & Mayuko Sato & Kiminori Toyooka & Hiroo Fukuda & Yoshihisa Oda, 2019. "A Rho-actin signaling pathway shapes cell wall boundaries in Arabidopsis xylem vessels," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    3. Annelie Carlsbecker & Ji-Young Lee & Christina J. Roberts & Jan Dettmer & Satu Lehesranta & Jing Zhou & Ove Lindgren & Miguel A. Moreno-Risueno & Anne Vatén & Siripong Thitamadee & Ana Campilho & Jose, 2010. "Cell signalling by microRNA165/6 directs gene dose-dependent root cell fate," Nature, Nature, vol. 465(7296), pages 316-321, May.
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