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Human ANP32A/B are SUMOylated and utilized by avian influenza virus NS2 protein to overcome species-specific restriction

Author

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  • Liuke Sun

    (The Chinese Academy of Agricultural Sciences)

  • Xing Guo

    (The Chinese Academy of Agricultural Sciences)

  • Mengmeng Yu

    (The Chinese Academy of Agricultural Sciences)

  • Xue-Feng Wang

    (The Chinese Academy of Agricultural Sciences)

  • Huiling Ren

    (The Chinese Academy of Agricultural Sciences)

  • Xiaojun Wang

    (The Chinese Academy of Agricultural Sciences
    The Chinese Academy of Agricultural Sciences)

Abstract

Human ANP32A/B (huANP32A/B) poorly support the polymerase activity of avian influenza viruses (AIVs), thereby limiting interspecies transmission of AIVs from birds to humans. The SUMO-interacting motif (SIM) within NS2 promotes the adaptation of AIV polymerase to huANP32A/B via a yet undisclosed mechanism. Here we show that huANP32A/B are SUMOylated by the E3 SUMO ligase PIAS2α, and deSUMOylated by SENP1. SUMO modification of huANP32A/B results in the recruitment of NS2, thereby facilitating huANP32A/B-supported AIV polymerase activity. Such a SUMO-dependent recruitment of NS2 is mediated by its association with huANP32A/B via the SIM-SUMO interaction module, where K68/K153-SUMO in huANP32A or K68/K116-SUMO in huANP32B interacts with the NS2-SIM. The SIM-SUMO-mediated interactions between NS2 and huANP32A/B function to promote AIV polymerase activity by positively regulating AIV vRNP-huANP32A/B interactions and AIV vRNP assembly. Our study offers insights into the mechanism of NS2-SIM in facilitating AIVs adaptation to mammals.

Suggested Citation

  • Liuke Sun & Xing Guo & Mengmeng Yu & Xue-Feng Wang & Huiling Ren & Xiaojun Wang, 2024. "Human ANP32A/B are SUMOylated and utilized by avian influenza virus NS2 protein to overcome species-specific restriction," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-55034-y
    DOI: 10.1038/s41467-024-55034-y
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    References listed on IDEAS

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    1. Ecco Staller & Loïc Carrique & Olivia C. Swann & Haitian Fan & Jeremy R. Keown & Carol M. Sheppard & Wendy S. Barclay & Jonathan M. Grimes & Ervin Fodor, 2024. "Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    2. Benjamin Mänz & Linda Brunotte & Peter Reuther & Martin Schwemmle, 2012. "Adaptive mutations in NEP compensate for defective H5N1 RNA replication in cultured human cells," Nature Communications, Nature, vol. 3(1), pages 1-11, January.
    3. Benoît Arragain & Tim Krischuns & Martin Pelosse & Petra Drncova & Martin Blackledge & Nadia Naffakh & Stephen Cusack, 2024. "Structures of influenza A and B replication complexes give insight into avian to human host adaptation and reveal a role of ANP32 as an electrostatic chaperone for the apo-polymerase," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
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