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Image-guided optogenetic spatiotemporal tissue patterning using μPatternScope

Author

Listed:
  • Sant Kumar

    (ETH Zürich)

  • Hannes M. Beyer

    (Heinrich-Heine-University Düsseldorf)

  • Mingzhe Chen

    (ETH Zürich)

  • Matias D. Zurbriggen

    (Heinrich-Heine-University Düsseldorf
    Düsseldorf)

  • Mustafa Khammash

    (ETH Zürich)

Abstract

In the field of tissue engineering, achieving precise spatiotemporal control over engineered cells is critical for sculpting functional 2D cell cultures into intricate morphological shapes. In this study, we engineer light-responsive mammalian cells and target them with dynamic light patterns to realize 2D cell culture patterning control. To achieve this, we developed μPatternScope (μPS), a modular framework for software-controlled projection of high-resolution light patterns onto microscope samples. μPS comprises hardware and software suite governing pattern projection and microscope maneuvers. Together with a 2D culture of the engineered cells, we utilize μPS for controlled spatiotemporal induction of apoptosis to generate desired 2D shapes. Furthermore, we introduce interactive closed-loop patterning, enabling a dynamic feedback mechanism between the measured cell culture patterns and the light illumination profiles to achieve the desired target patterning trends. Our work offers innovative tools for advanced tissue engineering applications through seamless fusion of optogenetics, optical engineering, and cybernetics.

Suggested Citation

  • Sant Kumar & Hannes M. Beyer & Mingzhe Chen & Matias D. Zurbriggen & Mustafa Khammash, 2024. "Image-guided optogenetic spatiotemporal tissue patterning using μPatternScope," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54351-6
    DOI: 10.1038/s41467-024-54351-6
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    References listed on IDEAS

    as
    1. Dirk Ollech & Tim Pflästerer & Adam Shellard & Chiara Zambarda & Joachim Pius Spatz & Philippe Marcq & Roberto Mayor & Richard Wombacher & Elisabetta Ada Cavalcanti-Adam, 2020. "Author Correction: An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells," Nature Communications, Nature, vol. 11(1), pages 1-1, December.
    2. Melinda Liu Perkins & Dirk Benzinger & Murat Arcak & Mustafa Khammash, 2020. "Cell-in-the-loop pattern formation with optogenetically emulated cell-to-cell signaling," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    3. Remy Chait & Jakob Ruess & Tobias Bergmiller & Gašper Tkačik & Călin C. Guet, 2017. "Shaping bacterial population behavior through computer-interfaced control of individual cells," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
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    5. Dirk Ollech & Tim Pflästerer & Adam Shellard & Chiara Zambarda & Joachim Pius Spatz & Philippe Marcq & Roberto Mayor & Richard Wombacher & Elisabetta Ada Cavalcanti-Adam, 2020. "An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
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