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Depolarization induces calcium-dependent BMP4 release from mouse embryonic palate mesenchymal cells

Author

Listed:
  • Mikaela L. Follmer

    (University of Colorado Anschutz Medical Campus)

  • Trevor J. Isner

    (University of Colorado Anschutz Medical Campus)

  • Yunus H. Ozekin

    (University of Colorado Anschutz Medical Campus)

  • Claire H. Levitt

    (University of Colorado Denver Anschutz Medical Campus)

  • Carolyn L. Burek

    (University of Colorado Anschutz Medical Campus)

  • Richard K. P. Benninger

    (University of Colorado Denver Anschutz Medical Campus)

  • Emily Anne Bates

    (University of Colorado Anschutz Medical Campus)

Abstract

Bone Morphogenetic Protein (BMP) signaling is essential for craniofacial development, though little is known about the mechanisms that govern BMP secretion. We show that depolarization induces calcium-dependent BMP4 release from mouse embryonic palate mesenchyme. We show endogenous transient changes in intracellular calcium occur in cranial neural crest cells, the cells from which embryonic palate mesenchyme derives. Waves of transient changes in intracellular calcium suggest that these cells are electrically coupled and may temporally coordinate BMP release. These transient changes in intracellular calcium persist in palate mesenchyme cells from embryonic day 9.5 to 13.5 mice. Disruption of a potassium channel called Kcnj2 significantly decreases the amplitude of calcium transients and the ability of cells to secrete BMP. Kcnj2 knockout mice have cleft palate and reduced BMP signaling. Our data suggest that temporal control of developmental cues is regulated by ion channels, depolarization, and intracellular calcium for mammalian craniofacial morphogenesis.

Suggested Citation

  • Mikaela L. Follmer & Trevor J. Isner & Yunus H. Ozekin & Claire H. Levitt & Carolyn L. Burek & Richard K. P. Benninger & Emily Anne Bates, 2024. "Depolarization induces calcium-dependent BMP4 release from mouse embryonic palate mesenchymal cells," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53642-2
    DOI: 10.1038/s41467-024-53642-2
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    1. Alessandro De Simone & Maya N. Evanitsky & Luke Hayden & Ben D. Cox & Julia Wang & Valerie A. Tornini & Jianhong Ou & Anna Chao & Kenneth D. Poss & Stefano Di Talia, 2021. "Control of osteoblast regeneration by a train of Erk activity waves," Nature, Nature, vol. 590(7844), pages 129-133, February.
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