IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-53322-1.html
   My bibliography  Save this article

Determinants of selectivity in the dicing mechanism

Author

Listed:
  • Thi Nhu-Y Le

    (The Hong Kong University of Science & Technology)

  • Cong Truc Le

    (The Hong Kong University of Science & Technology)

  • Tuan Anh Nguyen

    (The Hong Kong University of Science & Technology)

Abstract

Our research elucidates the cleavage processes of the RNase III enzyme, DICER, which plays a crucial role in the production of small RNAs, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs). Utilizing high-throughput dicing assays, we expose the bipartite pairing rule that dictates the cleavage sites of DICER. Furthermore, we decode the intricate recognition mechanism of the primary YCR motif and identify an analogous secondary YCR motif that influences DICER's cleavage choices. Collectively, our findings clarify the bipartite pairing rule and enhance our understanding of the role of RNA motifs in modulating DICER's cleavage activity, laying the groundwork for future research on their roles in miRNA biogenesis and gene regulation.

Suggested Citation

  • Thi Nhu-Y Le & Cong Truc Le & Tuan Anh Nguyen, 2024. "Determinants of selectivity in the dicing mechanism," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53322-1
    DOI: 10.1038/s41467-024-53322-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-53322-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-53322-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Trung Duc Nguyen & Tam Anh Trinh & Sheng Bao & Tuan Anh Nguyen, 2022. "Secondary structure RNA elements control the cleavage activity of DICER," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Jeffrey Vedanayagam & Walid K. Chatila & Bülent Arman Aksoy & Sonali Majumdar & Anders Jacobsen Skanderup & Emek Demir & Nikolaus Schultz & Chris Sander & Eric C. Lai, 2019. "Cancer-associated mutations in DICER1 RNase IIIa and IIIb domains exert similar effects on miRNA biogenesis," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    3. Young-Yoon Lee & Haedong Kim & V. Narry Kim, 2023. "Sequence determinant of small RNA production by DICER," Nature, Nature, vol. 615(7951), pages 323-330, March.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Trung Duc Nguyen & Tam Anh Trinh & Sheng Bao & Tuan Anh Nguyen, 2022. "Secondary structure RNA elements control the cleavage activity of DICER," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53322-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.