Author
Listed:
- Jeffrey Vedanayagam
(Memorial Sloan-Kettering Cancer Center)
- Walid K. Chatila
(Memorial Sloan-Kettering Cancer Center
Weill Cornell Medical College
Memorial Sloan Kettering Cancer Center)
- Bülent Arman Aksoy
(Memorial Sloan-Kettering Cancer Center
Weill Cornell Medical College
Medical University of South Carolina)
- Sonali Majumdar
(Memorial Sloan-Kettering Cancer Center)
- Anders Jacobsen Skanderup
(Memorial Sloan-Kettering Cancer Center
Genome Institute of Singapore)
- Emek Demir
(Memorial Sloan-Kettering Cancer Center
Computational Biology Program)
- Nikolaus Schultz
(Memorial Sloan Kettering Cancer Center
Memorial Sloan Kettering Cancer Center
Memorial Sloan Kettering Cancer Center)
- Chris Sander
(Memorial Sloan-Kettering Cancer Center
Dana-Farber Cancer Institute
Harvard Medical School)
- Eric C. Lai
(Memorial Sloan-Kettering Cancer Center
Weill Cornell Medical College)
Abstract
Somatic mutations in the RNase IIIb domain of DICER1 arise in cancer and disrupt the cleavage of 5' pre-miRNA arms. Here, we characterize an unstudied, recurrent, mutation (S1344L) in the DICER1 RNase IIIa domain in tumors from The Cancer Genome Atlas (TCGA) project and MSK-IMPACT profiling. RNase IIIa/b hotspots are absent from most cancers, but are notably enriched in uterine cancers. Systematic analysis of TCGA small RNA datasets show that DICER1 RNase IIIa-S1344L tumors deplete 5p-miRNAs, analogous to RNase IIIb hotspot samples. Structural and evolutionary coupling analyses reveal constrained proximity of RNase IIIa-S1344 to the RNase IIIb catalytic site, rationalizing why mutation of this site phenocopies known hotspot alterations. Finally, examination of DICER1 hotspot endometrial tumors reveals derepression of specific miRNA target signatures. In summary, comprehensive analyses of DICER1 somatic mutations and small RNA data reveal a mechanistic aspect of pre-miRNA processing that manifests in specific cancer settings.
Suggested Citation
Jeffrey Vedanayagam & Walid K. Chatila & Bülent Arman Aksoy & Sonali Majumdar & Anders Jacobsen Skanderup & Emek Demir & Nikolaus Schultz & Chris Sander & Eric C. Lai, 2019.
"Cancer-associated mutations in DICER1 RNase IIIa and IIIb domains exert similar effects on miRNA biogenesis,"
Nature Communications, Nature, vol. 10(1), pages 1-14, December.
Handle:
RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11610-1
DOI: 10.1038/s41467-019-11610-1
Download full text from publisher
Citations
Citations are extracted by the
CitEc Project, subscribe to its
RSS feed for this item.
Cited by:
- Trung Duc Nguyen & Tam Anh Trinh & Sheng Bao & Tuan Anh Nguyen, 2022.
"Secondary structure RNA elements control the cleavage activity of DICER,"
Nature Communications, Nature, vol. 13(1), pages 1-16, December.
- Thi Nhu-Y Le & Cong Truc Le & Tuan Anh Nguyen, 2024.
"Determinants of selectivity in the dicing mechanism,"
Nature Communications, Nature, vol. 15(1), pages 1-16, December.
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11610-1. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.