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Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli

Author

Listed:
  • Zhi-Qiang Xu

    (University of Wollongong)

  • Slobodan Jergic

    (University of Wollongong)

  • Allen T. Y. Lo

    (University of Wollongong)

  • Alok C. Pradhan

    (University of Wollongong
    University of Wollongong)

  • Simon H. J. Brown

    (University of Wollongong
    University of Wollongong)

  • James C. Bouwer

    (University of Wollongong
    University of Wollongong)

  • Harshad Ghodke

    (University of Wollongong)

  • Peter J. Lewis

    (University of Wollongong
    Hunter Biological Solutions)

  • Gökhan Tolun

    (University of Wollongong
    University of Wollongong)

  • Aaron J. Oakley

    (University of Wollongong)

  • Nicholas E. Dixon

    (University of Wollongong
    University of Wollongong)

Abstract

Ring-shaped DNA sliding clamps are essential for DNA replication and genome maintenance. Clamps need to be opened and chaperoned onto DNA by clamp loader complexes (CLCs). Detailed understanding of the mechanisms by which CLCs open and place clamps around DNA remains incomplete. Here, we present a series of six structures of the Escherichia coli CLC bound to an open or closed clamp prior to and after binding to a primer-template DNA, representing the most significant intermediates in the clamp loading process. We show that the ATP-bound CLC first binds to a clamp, then constricts to hold onto it. The CLC then expands to open the clamp with a gap large enough for double-stranded DNA to enter. Upon binding to DNA, the CLC constricts slightly, allowing clamp closing around DNA. These structures provide critical high-resolution snapshots of clamp loading by the E. coli CLC, revealing how the molecular machine works.

Suggested Citation

  • Zhi-Qiang Xu & Slobodan Jergic & Allen T. Y. Lo & Alok C. Pradhan & Simon H. J. Brown & James C. Bouwer & Harshad Ghodke & Peter J. Lewis & Gökhan Tolun & Aaron J. Oakley & Nicholas E. Dixon, 2024. "Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52623-9
    DOI: 10.1038/s41467-024-52623-9
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    References listed on IDEAS

    as
    1. Jiaquan Liu & Jeungphill Hanne & Brooke M. Britton & Jared Bennett & Daehyung Kim & Jong-Bong Lee & Richard Fishel, 2016. "Cascading MutS and MutL sliding clamps control DNA diffusion to activate mismatch repair," Nature, Nature, vol. 539(7630), pages 583-587, November.
    2. Xiao-Wen Yang & Xiao-Peng Han & Chong Han & James London & Richard Fishel & Jiaquan Liu, 2022. "MutS functions as a clamp loader by positioning MutL on the DNA during mismatch repair," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    3. Gregory D. Bowman & Mike O'Donnell & John Kuriyan, 2004. "Structural analysis of a eukaryotic sliding DNA clamp–clamp loader complex," Nature, Nature, vol. 429(6993), pages 724-730, June.
    Full references (including those not matched with items on IDEAS)

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