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Electron transfer in the respiratory chain at low salinity

Author

Listed:
  • Ana Paula Lobez

    (Stockholm University)

  • Fei Wu

    (Stockholm University)

  • Justin M. Di Trani

    (Toronto
    University of Alberta)

  • John L. Rubinstein

    (Toronto
    Toronto
    Toronto)

  • Mikael Oliveberg

    (Stockholm University)

  • Peter Brzezinski

    (Stockholm University)

  • Agnes Moe

    (Stockholm University
    Bühlstrasse 28)

Abstract

Recent studies have established that cellular electrostatic interactions are more influential than assumed previously. Here, we use cryo-EM and perform steady-state kinetic studies to investigate electrostatic interactions between cytochrome (cyt.) c and the complex (C) III2-IV supercomplex from Saccharomyces cerevisiae at low salinity. The kinetic studies show a sharp transition with a Hill coefficient ≥2, which together with the cryo-EM data at 2.4 Å resolution indicate multiple cyt. c molecules bound along the supercomplex surface. Negatively charged loops of CIII2 subunits Qcr6 and Qcr9 become structured to interact with cyt. c. In addition, the higher resolution allows us to identify water molecules in proton pathways of CIV and, to the best of our knowledge, previously unresolved cardiolipin molecules. In conclusion, the lowered electrostatic screening renders engagement of multiple cyt. c molecules that are directed by electrostatically structured CIII2 loops to conduct electron transfer between CIII2 and CIV.

Suggested Citation

  • Ana Paula Lobez & Fei Wu & Justin M. Di Trani & John L. Rubinstein & Mikael Oliveberg & Peter Brzezinski & Agnes Moe, 2024. "Electron transfer in the respiratory chain at low salinity," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52475-3
    DOI: 10.1038/s41467-024-52475-3
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    References listed on IDEAS

    as
    1. Corey F. Hryc & Venkata K. P. S. Mallampalli & Evgeniy I. Bovshik & Stavros Azinas & Guizhen Fan & Irina I. Serysheva & Genevieve C. Sparagna & Matthew L. Baker & Eugenia Mileykovskaya & William Dowha, 2023. "Structural insights into cardiolipin replacement by phosphatidylglycerol in a cardiolipin-lacking yeast respiratory supercomplex," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Wei-Chun Kao & Claire Ortmann de Percin Northumberland & Tat Cheung Cheng & Julio Ortiz & Alexandre Durand & Ottilie Loeffelholz & Oliver Schilling & Martin L. Biniossek & Bruno P. Klaholz & Carola Hu, 2022. "Structural basis for safe and efficient energy conversion in a respiratory supercomplex," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Irene Vercellino & Leonid A. Sazanov, 2021. "Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV," Nature, Nature, vol. 598(7880), pages 364-367, October.
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