IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-51091-5.html
   My bibliography  Save this article

Integration of shoot-derived polypeptide signals by root TGA transcription factors is essential for survival under fluctuating nitrogen environments

Author

Listed:
  • Ryutaro Kobayashi

    (Nagoya University)

  • Yuri Ohkubo

    (Nagoya University)

  • Mai Izumi

    (Nagoya University)

  • Ryosuke Ota

    (Nagoya University)

  • Keiko Yamada

    (Nagoya University)

  • Yoko Hayashi

    (Nagoya University)

  • Yasuko Yamashita

    (Nagoya University)

  • Saki Noda

    (Nagoya University)

  • Mari Ogawa-Ohnishi

    (Nagoya University)

  • Yoshikatsu Matsubayashi

    (Nagoya University)

Abstract

Unlike plants in the field, which experience significant temporal fluctuations in environmental conditions, plants in the laboratory are typically grown in controlled, stable environments. Therefore, signaling pathways evolved for survival in fluctuating environments often remain functionally latent in laboratory settings. Here, we show that TGA1 and TGA4 act as hub transcription factors through which the expression of genes involved in high-affinity nitrate uptake are regulated in response to shoot-derived phloem mobile polypeptides, CEP DOWNSTREAM 1 (CEPD1), CEPD2 and CEPD-like 2 (CEPDL2) as nitrogen (N) deficiency signals, and Glutaredoxin S1 (GrxS1) to GrxS8 as N sufficiency signals. CEPD1/2/CEPDL2 and GrxS1-S8 competitively bind to TGA1/4 in roots, with the former acting as transcription coactivators that enhance the uptake of nitrate, while the latter function as corepressor complexes together with TOPLESS (TPL), TPL-related 1 (TPR1) and TPR4 to limit nitrate uptake. Arabidopsis plants deficient in TGA1/4 maintain basal nitrate uptake and exhibit growth similar to wild-type plants in a stable N environment, but are impaired in regulation of nitrate acquisition in response to shoot N demand, leading to defective growth under fluctuating N environments where rhizosphere nitrate ions switch periodically between deficient and sufficient states. TGA1/4 are crucial transcription factors that enable plants to survive under fluctuating and challenging N environmental conditions.

Suggested Citation

  • Ryutaro Kobayashi & Yuri Ohkubo & Mai Izumi & Ryosuke Ota & Keiko Yamada & Yoko Hayashi & Yasuko Yamashita & Saki Noda & Mari Ogawa-Ohnishi & Yoshikatsu Matsubayashi, 2024. "Integration of shoot-derived polypeptide signals by root TGA transcription factors is essential for survival under fluctuating nitrogen environments," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51091-5
    DOI: 10.1038/s41467-024-51091-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-51091-5
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-024-51091-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Xiangchao Gan & Oliver Stegle & Jonas Behr & Joshua G. Steffen & Philipp Drewe & Katie L. Hildebrand & Rune Lyngsoe & Sebastian J. Schultheiss & Edward J. Osborne & Vipin T. Sreedharan & André Kahles , 2011. "Multiple reference genomes and transcriptomes for Arabidopsis thaliana," Nature, Nature, vol. 477(7365), pages 419-423, September.
    2. Ryosuke Ota & Yuri Ohkubo & Yasuko Yamashita & Mari Ogawa-Ohnishi & Yoshikatsu Matsubayashi, 2020. "Shoot-to-root mobile CEPD-like 2 integrates shoot nitrogen status to systemically regulate nitrate uptake in Arabidopsis," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Christina L Richards & Ulises Rosas & Joshua Banta & Naeha Bhambhra & Michael D Purugganan, 2012. "Genome-Wide Patterns of Arabidopsis Gene Expression in Nature," PLOS Genetics, Public Library of Science, vol. 8(4), pages 1-14, April.
    2. Moritz Sexauer & Hemal Bhasin & Maria Schön & Elena Roitsch & Caroline Wall & Ulrike Herzog & Katharina Markmann, 2023. "A micro RNA mediates shoot control of root branching," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    3. Tamer Aldwairi & David J. Chevalier & Andy D. Perkins, 2021. "Exploring the Effect of Climate Factors on SNPs within FHA Domain Genes in Eurasian Arabidopsis Ecotypes," Agriculture, MDPI, vol. 11(2), pages 1-10, February.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51091-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.