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tracerDB: a crowdsourced fluorescent tracer database for target engagement analysis

Author

Listed:
  • Johannes Dopfer

    (Goethe University Frankfurt
    Buchmann Institute for Life Sciences)

  • James D. Vasta

    (Promega Corporation)

  • Susanne Müller

    (Goethe University Frankfurt
    Buchmann Institute for Life Sciences)

  • Stefan Knapp

    (Goethe University Frankfurt
    Buchmann Institute for Life Sciences
    DKTK Site Frankfurt-Mainz)

  • Matthew B. Robers

    (Promega Corporation)

  • Martin P. Schwalm

    (Goethe University Frankfurt
    Buchmann Institute for Life Sciences
    DKTK Site Frankfurt-Mainz)

Abstract

Investigating ligand-protein complexes is essential in the areas of chemical biology and drug discovery. However, detailed information on key reagents such as fluorescent tracers and associated data for the development of widely used bioluminescence resonance energy transfer (BRET) assays including NanoBRET, time-resolved Förster resonance energy transfer (TR-FRET) and fluorescence polarization (FP) assays are not easily accessible to the research community. We created tracerDB, a curated database of validated tracers. This resource provides an open access knowledge base and a unified system for tracer and assay validation. The database is freely available at https://www.tracerdb.org/ .

Suggested Citation

  • Johannes Dopfer & James D. Vasta & Susanne Müller & Stefan Knapp & Matthew B. Robers & Martin P. Schwalm, 2024. "tracerDB: a crowdsourced fluorescent tracer database for target engagement analysis," Nature Communications, Nature, vol. 15(1), pages 1-5, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49896-5
    DOI: 10.1038/s41467-024-49896-5
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    References listed on IDEAS

    as
    1. Matthew B. Robers & Melanie L. Dart & Carolyn C. Woodroofe & Chad A. Zimprich & Thomas A. Kirkland & Thomas Machleidt & Kevin R. Kupcho & Sergiy Levin & James R. Hartnett & Kristopher Zimmerman & Andr, 2015. "Target engagement and drug residence time can be observed in living cells with BRET," Nature Communications, Nature, vol. 6(1), pages 1-10, December.
    2. Jonathan Baell & Michael A. Walters, 2014. "Chemistry: Chemical con artists foil drug discovery," Nature, Nature, vol. 513(7519), pages 481-483, September.
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