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m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation

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  • Pierre Klein

    (Darwin Building
    The Francis Crick Institute)

  • Marija Petrić Howe

    (The Francis Crick Institute
    University College London)

  • Jasmine Harley

    (The Francis Crick Institute
    University College London)

  • Harry Crook

    (The Francis Crick Institute)

  • Sofia Esteban Serna

    (Darwin Building)

  • Theodoros I. Roumeliotis

    (The Institute of Cancer Research)

  • Jyoti S. Choudhary

    (The Institute of Cancer Research)

  • Anob M. Chakrabarti

    (The Francis Crick Institute)

  • Raphaëlle Luisier

    (Idiap Research Institute
    SIB Swiss Institute of Bioinformatics)

  • Rickie Patani

    (The Francis Crick Institute
    University College London)

  • Andres Ramos

    (Darwin Building)

Abstract

Neuronal differentiation requires building a complex intracellular architecture, and therefore the coordinated regulation of defined sets of genes. RNA-binding proteins (RBPs) play a key role in this regulation. However, while their action on individual mRNAs has been explored in depth, the mechanisms used to coordinate gene expression programs shaping neuronal morphology are poorly understood. To address this, we studied how the paradigmatic RBP IMP1 (IGF2BP1), an essential developmental factor, selects and regulates its RNA targets during the human neuronal differentiation. We perform a combination of system-wide and molecular analyses, revealing that IMP1 developmentally transitions to and directly regulates the expression of mRNAs encoding essential regulators of the microtubule network, a key component of neuronal morphology. Furthermore, we show that m6A methylation drives the selection of specific IMP1 mRNA targets and their protein expression during the developmental transition from neural precursors to neurons, providing a molecular principle for the onset of target selectivity.

Suggested Citation

  • Pierre Klein & Marija Petrić Howe & Jasmine Harley & Harry Crook & Sofia Esteban Serna & Theodoros I. Roumeliotis & Jyoti S. Choudhary & Anob M. Chakrabarti & Raphaëlle Luisier & Rickie Patani & Andre, 2024. "m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49139-7
    DOI: 10.1038/s41467-024-49139-7
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