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Drug screening on digital microfluidics for cancer precision medicine

Author

Listed:
  • Jiao Zhai

    (University of Macau
    City University of Hong Kong)

  • Yingying Liu

    (University of Macau
    University of Macau)

  • Weiqing Ji

    (University of Science and Technology Beijing)

  • Xinru Huang

    (Sun Yat-Sen University)

  • Ping Wang

    (The First Affiliated Hospital of Guangzhou Medical University)

  • Yunyi Li

    (University of Macau)

  • Haoran Li

    (University of Macau
    University of Macau)

  • Ada Hang-Heng Wong

    (University of Macau)

  • Xiong Zhou

    (University of Macau
    Hunan University)

  • Ping Chen

    (University of Macau)

  • Lianhong Wang

    (Hunan University)

  • Ning Yang

    (University of Macau
    Jiangsu University)

  • Chi Chen

    (Sun Yat-Sen University)

  • Haitian Chen

    (Sun Yat-Sen University)

  • Pui-In Mak

    (University of Macau
    University of Macau)

  • Chu-Xia Deng

    (University of Macau)

  • Rui Martins

    (University of Macau
    University of Macau
    Universidade de Lisboa)

  • Mengsu Yang

    (City University of Hong Kong)

  • Tsung-Yi Ho

    (The Chinese University of Hong Kong)

  • Shuhong Yi

    (Sun Yat-Sen University)

  • Hailong Yao

    (University of Science and Technology Beijing)

  • Yanwei Jia

    (University of Macau
    University of Macau
    University of Macau)

Abstract

Drug screening based on in-vitro primary tumor cell culture has demonstrated potential in personalized cancer diagnosis. However, the limited number of tumor cells, especially from patients with early stage cancer, has hindered the widespread application of this technique. Hence, we developed a digital microfluidic system for drug screening using primary tumor cells and established a working protocol for precision medicine. Smart control logic was developed to increase the throughput of the system and decrease its footprint to parallelly screen three drugs on a 4 × 4 cm2 chip in a device measuring 23 × 16 × 3.5 cm3. We validated this method in an MDA-MB-231 breast cancer xenograft mouse model and liver cancer specimens from patients, demonstrating tumor suppression in mice/patients treated with drugs that were screened to be effective on individual primary tumor cells. Mice treated with drugs screened on-chip as ineffective exhibited similar results to those in the control groups. The effective drug identified through on-chip screening demonstrated consistency with the absence of mutations in their related genes determined via exome sequencing of individual tumors, further validating this protocol. Therefore, this technique and system may promote advances in precision medicine for cancer treatment and, eventually, for any disease.

Suggested Citation

  • Jiao Zhai & Yingying Liu & Weiqing Ji & Xinru Huang & Ping Wang & Yunyi Li & Haoran Li & Ada Hang-Heng Wong & Xiong Zhou & Ping Chen & Lianhong Wang & Ning Yang & Chi Chen & Haitian Chen & Pui-In Mak , 2024. "Drug screening on digital microfluidics for cancer precision medicine," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48616-3
    DOI: 10.1038/s41467-024-48616-3
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    References listed on IDEAS

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    1. Mary V. Relling & William E. Evans, 2015. "Pharmacogenomics in the clinic," Nature, Nature, vol. 526(7573), pages 343-350, October.
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