IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-46565-5.html
   My bibliography  Save this article

Lipoarabinomannan mediates localized cell wall integrity during division in mycobacteria

Author

Listed:
  • Ian L. Sparks

    (University of Massachusetts)

  • Takehiro Kado

    (University of Massachusetts)

  • Malavika Prithviraj

    (University of Massachusetts)

  • Japinder Nijjer

    (Yale University
    Yale University)

  • Jing Yan

    (Yale University
    Yale University)

  • Yasu S. Morita

    (University of Massachusetts)

Abstract

The growth and division of mycobacteria, which include clinically relevant pathogens, deviate from that of canonical bacterial models. Despite their Gram-positive ancestry, mycobacteria synthesize and elongate a diderm envelope asymmetrically from the poles, with the old pole elongating more robustly than the new pole. The phosphatidylinositol-anchored lipoglycans lipomannan (LM) and lipoarabinomannan (LAM) are cell envelope components critical for host-pathogen interactions, but their physiological functions in mycobacteria remained elusive. In this work, using biosynthetic mutants of these lipoglycans, we examine their roles in maintaining cell envelope integrity in Mycobacterium smegmatis and Mycobacterium tuberculosis. We find that mutants defective in producing mature LAM fail to maintain rod cell shape specifically at the new pole and para-septal regions whereas a mutant that produces a larger LAM becomes multi-septated. Therefore, LAM plays critical and distinct roles at subcellular locations associated with division in mycobacteria, including maintenance of local cell wall integrity and septal placement.

Suggested Citation

  • Ian L. Sparks & Takehiro Kado & Malavika Prithviraj & Japinder Nijjer & Jing Yan & Yasu S. Morita, 2024. "Lipoarabinomannan mediates localized cell wall integrity during division in mycobacteria," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46565-5
    DOI: 10.1038/s41467-024-46565-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-46565-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-46565-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Isabella Santi & Neeraj Dhar & Djenet Bousbaine & Yuichi Wakamoto & John D. McKinney, 2013. "Erratum: Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria," Nature Communications, Nature, vol. 4(1), pages 1-1, December.
    2. Isabella Santi & Neeraj Dhar & Djenet Bousbaine & Yuichi Wakamoto & John D. McKinney, 2013. "Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria," Nature Communications, Nature, vol. 4(1), pages 1-11, December.
    3. E. Hesper Rego & Rebecca E. Audette & Eric J. Rubin, 2017. "Deletion of a mycobacterial divisome factor collapses single-cell phenotypic heterogeneity," Nature, Nature, vol. 546(7656), pages 153-157, June.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ana Teresa López-Jiménez & Serge Mostowy, 2021. "Emerging technologies and infection models in cellular microbiology," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    2. Kaj M. Kreutzfeldt & Robert S. Jansen & Travis E. Hartman & Alexandre Gouzy & Ruojun Wang & Inna V. Krieger & Matthew D. Zimmerman & Martin Gengenbacher & Jansy P. Sarathy & Min Xie & Véronique Dartoi, 2022. "CinA mediates multidrug tolerance in Mycobacterium tuberculosis," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    3. Maxime Mistretta & Mena Cimino & Pascal Campagne & Stevenn Volant & Etienne Kornobis & Olivier Hebert & Christophe Rochais & Patrick Dallemagne & Cédric Lecoutey & Camille Tisnerat & Alban Lepailleur , 2024. "Dynamic microfluidic single-cell screening identifies pheno-tuning compounds to potentiate tuberculosis therapy," Nature Communications, Nature, vol. 15(1), pages 1-22, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46565-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.