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Lipoarabinomannan mediates localized cell wall integrity during division in mycobacteria

Author

Listed:
  • Ian L. Sparks

    (University of Massachusetts)

  • Takehiro Kado

    (University of Massachusetts)

  • Malavika Prithviraj

    (University of Massachusetts)

  • Japinder Nijjer

    (Yale University
    Yale University)

  • Jing Yan

    (Yale University
    Yale University)

  • Yasu S. Morita

    (University of Massachusetts)

Abstract

The growth and division of mycobacteria, which include clinically relevant pathogens, deviate from that of canonical bacterial models. Despite their Gram-positive ancestry, mycobacteria synthesize and elongate a diderm envelope asymmetrically from the poles, with the old pole elongating more robustly than the new pole. The phosphatidylinositol-anchored lipoglycans lipomannan (LM) and lipoarabinomannan (LAM) are cell envelope components critical for host-pathogen interactions, but their physiological functions in mycobacteria remained elusive. In this work, using biosynthetic mutants of these lipoglycans, we examine their roles in maintaining cell envelope integrity in Mycobacterium smegmatis and Mycobacterium tuberculosis. We find that mutants defective in producing mature LAM fail to maintain rod cell shape specifically at the new pole and para-septal regions whereas a mutant that produces a larger LAM becomes multi-septated. Therefore, LAM plays critical and distinct roles at subcellular locations associated with division in mycobacteria, including maintenance of local cell wall integrity and septal placement.

Suggested Citation

  • Ian L. Sparks & Takehiro Kado & Malavika Prithviraj & Japinder Nijjer & Jing Yan & Yasu S. Morita, 2024. "Lipoarabinomannan mediates localized cell wall integrity during division in mycobacteria," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46565-5
    DOI: 10.1038/s41467-024-46565-5
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    References listed on IDEAS

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    1. Isabella Santi & Neeraj Dhar & Djenet Bousbaine & Yuichi Wakamoto & John D. McKinney, 2013. "Erratum: Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria," Nature Communications, Nature, vol. 4(1), pages 1-1, December.
    2. Isabella Santi & Neeraj Dhar & Djenet Bousbaine & Yuichi Wakamoto & John D. McKinney, 2013. "Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria," Nature Communications, Nature, vol. 4(1), pages 1-11, December.
    3. E. Hesper Rego & Rebecca E. Audette & Eric J. Rubin, 2017. "Deletion of a mycobacterial divisome factor collapses single-cell phenotypic heterogeneity," Nature, Nature, vol. 546(7656), pages 153-157, June.
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    Cited by:

    1. Aaron Franklin & Vivian C. Salgueiro & Abigail J. Layton & Rudi Sullivan & Todd Mize & Lucía Vázquez-Iniesta & Samuel T. Benedict & Sudagar S. Gurcha & Itxaso Anso & Gurdyal S. Besra & Manuel Banzhaf , 2024. "The mycobacterial glycoside hydrolase LamH enables capsular arabinomannan release and stimulates growth," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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