IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-45710-4.html
   My bibliography  Save this article

A microfluidic platform integrating functional vascularized organoids-on-chip

Author

Listed:
  • Clément Quintard

    (Univ. Grenoble Alpes, CEA, IRIG/BGE, BIOMICS
    Univ. Grenoble Alpes, CEA, LETI, DTBS
    University of British Columbia)

  • Emily Tubbs

    (Univ. Grenoble Alpes, CEA, IRIG/BGE, BIOMICS)

  • Gustav Jonsson

    (Institute of Molecular Biotechnology of the Austrian Academy of Sciences, IMBA
    Doctoral School of the University of Vienna and Medical University of Vienna
    Medical University of Vienna)

  • Jie Jiao

    (University of British Columbia)

  • Jun Wang

    (University of British Columbia)

  • Nicolas Werschler

    (University of British Columbia)

  • Camille Laporte

    (Univ. Grenoble Alpes, CEA, IRIG/BGE, BIOMICS
    Univ. Grenoble Alpes, CEA, LETI, DTBS)

  • Amandine Pitaval

    (Univ. Grenoble Alpes, CEA, IRIG/BGE, BIOMICS)

  • Thierno-Sidy Bah

    (Univ. Grenoble Alpes, CEA, IRIG, BGE, Gen&Chem)

  • Gideon Pomeranz

    (UCL Great Ormond Street Institute of Child Health)

  • Caroline Bissardon

    (Univ. Grenoble Alpes, CEA, LETI, DTBS)

  • Joris Kaal

    (Univ. Grenoble Alpes, CEA, LETI, DTBS)

  • Alexandra Leopoldi

    (Institute of Molecular Biotechnology of the Austrian Academy of Sciences, IMBA
    Medical University of Vienna)

  • David A. Long

    (UCL Great Ormond Street Institute of Child Health)

  • Pierre Blandin

    (Univ. Grenoble Alpes, CEA, LETI, DTBS)

  • Jean-Luc Achard

    (Université Grenoble Alpes, CNRS, Grenoble INP, LEGI)

  • Christophe Battail

    (Univ. Grenoble Alpes, CEA, IRIG, BGE, Gen&Chem)

  • Astrid Hagelkruys

    (Institute of Molecular Biotechnology of the Austrian Academy of Sciences, IMBA
    Medical University of Vienna)

  • Fabrice Navarro

    (Univ. Grenoble Alpes, CEA, LETI, DTBS)

  • Yves Fouillet

    (Univ. Grenoble Alpes, CEA, LETI, DTBS)

  • Josef M. Penninger

    (University of British Columbia
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, IMBA
    Medical University of Vienna
    Helmholtz Centre for Infection Research)

  • Xavier Gidrol

    (Univ. Grenoble Alpes, CEA, IRIG/BGE, BIOMICS)

Abstract

The development of vascular networks in microfluidic chips is crucial for the long-term culture of three-dimensional cell aggregates such as spheroids, organoids, tumoroids, or tissue explants. Despite rapid advancement in microvascular network systems and organoid technologies, vascularizing organoids-on-chips remains a challenge in tissue engineering. Most existing microfluidic devices poorly reflect the complexity of in vivo flows and require complex technical set-ups. Considering these constraints, we develop a platform to establish and monitor the formation of endothelial networks around mesenchymal and pancreatic islet spheroids, as well as blood vessel organoids generated from pluripotent stem cells, cultured for up to 30 days on-chip. We show that these networks establish functional connections with the endothelium-rich spheroids and vascular organoids, as they successfully provide intravascular perfusion to these structures. We find that organoid growth, maturation, and function are enhanced when cultured on-chip using our vascularization method. This microphysiological system represents a viable organ-on-chip model to vascularize diverse biological 3D tissues and sets the stage to establish organoid perfusions using advanced microfluidics.

Suggested Citation

  • Clément Quintard & Emily Tubbs & Gustav Jonsson & Jie Jiao & Jun Wang & Nicolas Werschler & Camille Laporte & Amandine Pitaval & Thierno-Sidy Bah & Gideon Pomeranz & Caroline Bissardon & Joris Kaal & , 2024. "A microfluidic platform integrating functional vascularized organoids-on-chip," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45710-4
    DOI: 10.1038/s41467-024-45710-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-45710-4
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-024-45710-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Reiner A. Wimmer & Alexandra Leopoldi & Martin Aichinger & Nikolaus Wick & Brigitte Hantusch & Maria Novatchkova & Jasmin Taubenschmid & Monika Hämmerle & Christopher Esk & Joshua A. Bagley & Dominik , 2019. "Human blood vessel organoids as a model of diabetic vasculopathy," Nature, Nature, vol. 565(7740), pages 505-510, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Thomas L. Maurissen & Alena J. Spielmann & Gabriella Schellenberg & Marc Bickle & Jose Ricardo Vieira & Si Ying Lai & Georgios Pavlou & Sascha Fauser & Peter D. Westenskow & Roger D. Kamm & Héloïse Ra, 2024. "Modeling early pathophysiological phenotypes of diabetic retinopathy in a human inner blood-retinal barrier-on-a-chip," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Alexandru Achim & Agata Stanek & Călin Homorodean & Mihail Spinu & Horea Laurenţiu Onea & Leontin Lazăr & Mădălin Marc & Zoltán Ruzsa & Dan Mircea Olinic, 2022. "Approaches to Peripheral Artery Disease in Diabetes: Are There Any Differences?," IJERPH, MDPI, vol. 19(16), pages 1-11, August.
    3. Vanessa Monteil & Hyesoo Kwon & Lijo John & Cristiano Salata & Gustav Jonsson & Sabine U. Vorrink & Sofia Appelberg & Sonia Youhanna & Matheus Dyczynski & Alexandra Leopoldi & Nicole Leeb & Jennifer V, 2023. "Identification of CCZ1 as an essential lysosomal trafficking regulator in Marburg and Ebola virus infections," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Sara G. Romeo & Ilaria Secco & Edoardo Schneider & Christina M. Reumiller & Celio X. C. Santos & Anna Zoccarato & Vishal Musale & Aman Pooni & Xiaoke Yin & Konstantinos Theofilatos & Silvia Cellone Tr, 2023. "Human blood vessel organoids reveal a critical role for CTGF in maintaining microvascular integrity," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45710-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.