IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-44696-3.html
   My bibliography  Save this article

Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum

Author

Listed:
  • Rui Xu

    (Washington University School of Medicine
    South China Agricultural University)

  • Wandy L. Beatty

    (Washington University School of Medicine)

  • Valentin Greigert

    (Washington University School of Medicine)

  • William H. Witola

    (University of Illinois Urbana-Champaign)

  • L. David Sibley

    (Washington University School of Medicine)

Abstract

Cryptosporidium parvum is an obligate intracellular parasite with a highly reduced mitochondrion that lacks the tricarboxylic acid cycle and the ability to generate ATP, making the parasite reliant on glycolysis. Genetic ablation experiments demonstrated that neither of the two putative glucose transporters CpGT1 and CpGT2 were essential for growth. Surprisingly, hexokinase was also dispensable for parasite growth while the downstream enzyme aldolase was required, suggesting the parasite has an alternative way of obtaining phosphorylated hexose. Complementation studies in E. coli support a role for direct transport of glucose-6-phosphate from the host cell by the parasite transporters CpGT1 and CpGT2, thus bypassing a requirement for hexokinase. Additionally, the parasite obtains phosphorylated glucose from amylopectin stores that are released by the action of the essential enzyme glycogen phosphorylase. Collectively, these findings reveal that C. parvum relies on multiple pathways to obtain phosphorylated glucose both for glycolysis and to restore carbohydrate reserves.

Suggested Citation

  • Rui Xu & Wandy L. Beatty & Valentin Greigert & William H. Witola & L. David Sibley, 2024. "Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-44696-3
    DOI: 10.1038/s41467-024-44696-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-44696-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-44696-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Ping Xu & Giovanni Widmer & Yingping Wang & Luiz S. Ozaki & Joao M. Alves & Myrna G. Serrano & Daniela Puiu & Patricio Manque & Donna Akiyoshi & Aaron J. Mackey & William R. Pearson & Paul H. Dear & A, 2004. "Correction: Corrigendum: The genome of Cryptosporidium hominis," Nature, Nature, vol. 432(7015), pages 415-415, November.
    2. Ping Xu & Giovanni Widmer & Yingping Wang & Luiz S. Ozaki & Joao M. Alves & Myrna G. Serrano & Daniela Puiu & Patricio Manque & Donna Akiyoshi & Aaron J. Mackey & William R. Pearson & Paul H. Dear & A, 2004. "The genome of Cryptosporidium hominis," Nature, Nature, vol. 431(7012), pages 1107-1112, October.
    3. Casey M. Theriot & Mark J. Koenigsknecht & Paul E. Carlson & Gabrielle E. Hatton & Adam M. Nelson & Bo Li & Gary B. Huffnagle & Jun Z. Li & Vincent B. Young, 2014. "Antibiotic-induced shifts in the mouse gut microbiome and metabolome increase susceptibility to Clostridium difficile infection," Nature Communications, Nature, vol. 5(1), pages 1-10, May.
    4. Phuong N. Tran & Simon H. J. Brown & Todd W. Mitchell & Kai Matuschewski & Paul J. McMillan & Kiaran Kirk & Matthew W. A. Dixon & Alexander G. Maier, 2014. "A female gametocyte-specific ABC transporter plays a role in lipid metabolism in the malaria parasite," Nature Communications, Nature, vol. 5(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Corey C. Holt & Elisabeth Hehenberger & Denis V. Tikhonenkov & Victoria K. L. Jacko-Reynolds & Noriko Okamoto & Elizabeth C. Cooney & Nicholas A. T. Irwin & Patrick J. Keeling, 2023. "Multiple parallel origins of parasitic Marine Alveolates," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Emily C Woods & Adrianne N Edwards & Kevin O Childress & Joshua B Jones & Shonna M McBride, 2018. "The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37," PLOS Pathogens, Public Library of Science, vol. 14(8), pages 1-28, August.
    3. Alexander Y. G. Yip & Olivia G. King & Oleksii Omelchenko & Sanjana Kurkimat & Victoria Horrocks & Phoebe Mostyn & Nathan Danckert & Rohma Ghani & Giovanni Satta & Elita Jauneikaite & Frances J. Davie, 2023. "Antibiotics promote intestinal growth of carbapenem-resistant Enterobacteriaceae by enriching nutrients and depleting microbial metabolites," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    4. José A Fernández Robledo & Gerardo R Vasta & Nicholas R Record, 2014. "Protozoan Parasites of Bivalve Molluscs: Literature Follows Culture," PLOS ONE, Public Library of Science, vol. 9(6), pages 1-9, June.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-44696-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.