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MCT1-governed pyruvate metabolism is essential for antibody class-switch recombination through H3K27 acetylation

Author

Listed:
  • Wenna Chi

    (Tsinghua University
    Sichuan University)

  • Na Kang

    (Tsinghua University
    Tsinghua-Peking Center for Life Sciences)

  • Linlin Sheng

    (Tsinghua University)

  • Sichen Liu

    (Tsinghua University)

  • Lei Tao

    (Tsinghua University
    Sichuan University)

  • Xizhi Cao

    (Tsinghua University)

  • Ye Liu

    (Tsinghua University)

  • Can Zhu

    (Tsinghua University)

  • Yuming Zhang

    (Tsinghua University)

  • Bolong Wu

    (Tsinghua University)

  • Ruiqun Chen

    (Tsinghua University)

  • Lili Cheng

    (Tsinghua University)

  • Jing Wang

    (Tsinghua University)

  • Xiaolin Sun

    (Peking University People’s Hospital
    Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135))

  • Xiaohui Liu

    (Tsinghua University)

  • Haiteng Deng

    (Tsinghua University)

  • Jinliang Yang

    (Sichuan University)

  • Zhanguo Li

    (Tsinghua-Peking Center for Life Sciences
    Peking University People’s Hospital
    Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135))

  • Wanli Liu

    (Tsinghua University
    Tsinghua-Peking Center for Life Sciences)

  • Ligong Chen

    (Tsinghua University
    Sichuan University)

Abstract

Monocarboxylate transporter 1 (MCT1) exhibits essential roles in cellular metabolism and energy supply. Although MCT1 is highly expressed in activated B cells, it is not clear how MCT1-governed monocarboxylates transportation is functionally coupled to antibody production during the glucose metabolism. Here, we report that B cell-lineage deficiency of MCT1 significantly influences the class-switch recombination (CSR), rendering impaired IgG antibody responses in Mct1f/fMb1Cre mice after immunization. Metabolic flux reveals that glucose metabolism is significantly reprogrammed from glycolysis to oxidative phosphorylation in Mct1-deficient B cells upon activation. Consistently, activation-induced cytidine deaminase (AID), is severely suppressed in Mct1-deficient B cells due to the decreased level of pyruvate metabolite. Mechanistically, MCT1 is required to maintain the optimal concentration of pyruvate to secure the sufficient acetylation of H3K27 for the elevated transcription of AID in activated B cells. Clinically, we found that MCT1 expression levels are significantly upregulated in systemic lupus erythematosus patients, and Mct1 deficiency can alleviate the symptoms of bm12-induced murine lupus model. Collectively, these results demonstrate that MCT1-mediated pyruvate metabolism is required for IgG antibody CSR through an epigenetic dependent AID transcription, revealing MCT1 as a potential target for vaccine development and SLE disease treatment.

Suggested Citation

  • Wenna Chi & Na Kang & Linlin Sheng & Sichen Liu & Lei Tao & Xizhi Cao & Ye Liu & Can Zhu & Yuming Zhang & Bolong Wu & Ruiqun Chen & Lili Cheng & Jing Wang & Xiaolin Sun & Xiaohui Liu & Haiteng Deng & , 2024. "MCT1-governed pyruvate metabolism is essential for antibody class-switch recombination through H3K27 acetylation," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44540-0
    DOI: 10.1038/s41467-023-44540-0
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    References listed on IDEAS

    as
    1. Sung Hoon Cho & Ariel L. Raybuck & Kristy Stengel & Mei Wei & Thomas C. Beck & Emmanuel Volanakis & James W. Thomas & Scott Hiebert & Volker H. Haase & Mark R. Boothby, 2016. "Germinal centre hypoxia and regulation of antibody qualities by a hypoxia response system," Nature, Nature, vol. 537(7619), pages 234-238, September.
    2. Helia N. Sanchez & Justin B. Moroney & Huoqun Gan & Tian Shen & John L. Im & Tianbao Li & Julia R. Taylor & Hong Zan & Paolo Casali, 2020. "B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids," Nature Communications, Nature, vol. 11(1), pages 1-19, December.
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