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iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy

Author

Listed:
  • Vincent Louvel

    (University of Geneva)

  • Romuald Haase

    (University of Geneva)

  • Olivier Mercey

    (University of Geneva)

  • Marine H. Laporte

    (University of Geneva)

  • Thibaut Eloy

    (University of Strasbourg)

  • Étienne Baudrier

    (University of Strasbourg)

  • Denis Fortun

    (University of Strasbourg)

  • Dominique Soldati-Favre

    (University of Geneva)

  • Virginie Hamel

    (University of Geneva)

  • Paul Guichard

    (University of Geneva)

Abstract

Expansion microscopy (ExM) is a highly effective technique for super-resolution fluorescence microscopy that enables imaging of biological samples beyond the diffraction limit with conventional fluorescence microscopes. Despite the development of several enhanced protocols, ExM has not yet demonstrated the ability to achieve the precision of nanoscopy techniques such as Single Molecule Localization Microscopy (SMLM). Here, to address this limitation, we have developed an iterative ultrastructure expansion microscopy (iU-ExM) approach that achieves SMLM-level resolution. With iU-ExM, it is now possible to visualize the molecular architecture of gold-standard samples, such as the eight-fold symmetry of nuclear pores or the molecular organization of the conoid in Apicomplexa. With its wide-ranging applications, from isolated organelles to cells and tissue, iU-ExM opens new super-resolution avenues for scientists studying biological structures and functions.

Suggested Citation

  • Vincent Louvel & Romuald Haase & Olivier Mercey & Marine H. Laporte & Thibaut Eloy & Étienne Baudrier & Denis Fortun & Dominique Soldati-Favre & Virginie Hamel & Paul Guichard, 2023. "iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43582-8
    DOI: 10.1038/s41467-023-43582-8
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    References listed on IDEAS

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    1. Long Gui & William J. O’Shaughnessy & Kai Cai & Evan Reetz & Michael L. Reese & Daniela Nicastro, 2023. "Cryo-tomography reveals rigid-body motion and organization of apicomplexan invasion machinery," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Ons M’Saad & Joerg Bewersdorf, 2020. "Light microscopy of proteins in their ultrastructural context," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    3. Alexander von Appen & Jan Kosinski & Lenore Sparks & Alessandro Ori & Amanda L. DiGuilio & Benjamin Vollmer & Marie-Therese Mackmull & Niccolo Banterle & Luca Parca & Panagiotis Kastritis & Katarzyna , 2015. "In situ structural analysis of the human nuclear pore complex," Nature, Nature, vol. 526(7571), pages 140-143, October.
    4. Fabian U. Zwettler & Sebastian Reinhard & Davide Gambarotto & Toby D. M. Bell & Virginie Hamel & Paul Guichard & Markus Sauer, 2020. "Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
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    1. Andreas Müller & Nikolai Klena & Song Pang & Leticia Elizabeth Galicia Garcia & Oleksandra Topcheva & Solange Aurrecoechea Duran & Davud Sulaymankhil & Monika Seliskar & Hassan Mziaut & Eyke Schöniger, 2024. "Structure, interaction and nervous connectivity of beta cell primary cilia," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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