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Imaging cellular forces with photonic crystals

Author

Listed:
  • Qiwei Li

    (Southeast University)

  • Zaozao Chen

    (Southeast University
    Southeast University)

  • Ying Zhang

    (Southeast University)

  • Shuang Ding

    (Southeast University)

  • Haibo Ding

    (Southeast University)

  • Luping Wang

    (Southeast University
    Ningbo University)

  • Zhuoying Xie

    (Southeast University)

  • Yifu Fu

    (Southeast University)

  • Mengxiao Wei

    (Southeast University)

  • Shengnan Liu

    (Southeast University)

  • Jialun Chen

    (Southeast University)

  • Xuan Wang

    (Southeast University)

  • Zhongze Gu

    (Southeast University
    Southeast University)

Abstract

Current techniques for visualizing and quantifying cellular forces have limitations in live cell imaging, throughput, and multi-scale analysis, which impede progress in cell force research and its practical applications. We developed a photonic crystal cellular force microscopy (PCCFM) to image vertical cell forces over a wide field of view (1.3 mm ⨯ 1.0 mm, a 10 ⨯ objective image) at high speed (about 20 frames per second) without references. The photonic crystal hydrogel substrate (PCS) converts micro-nano deformations into perceivable color changes, enabling in situ visualization and quantification of tiny vertical cell forces with high throughput. It enabled long-term, cross-scale monitoring from subcellular focal adhesions to tissue-level cell sheets and aggregates.

Suggested Citation

  • Qiwei Li & Zaozao Chen & Ying Zhang & Shuang Ding & Haibo Ding & Luping Wang & Zhuoying Xie & Yifu Fu & Mengxiao Wei & Shengnan Liu & Jialun Chen & Xuan Wang & Zhongze Gu, 2023. "Imaging cellular forces with photonic crystals," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43090-9
    DOI: 10.1038/s41467-023-43090-9
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    References listed on IDEAS

    as
    1. Jérémie Teyssier & Suzanne V. Saenko & Dirk van der Marel & Michel C. Milinkovitch, 2015. "Photonic crystals cause active colour change in chameleons," Nature Communications, Nature, vol. 6(1), pages 1-7, May.
    2. Eyal Karzbrun & Aimal H. Khankhel & Heitor C. Megale & Stella M. K. Glasauer & Yofiel Wyle & George Britton & Aryeh Warmflash & Kenneth S. Kosik & Eric D. Siggia & Boris I. Shraiman & Sebastian J. Str, 2021. "Human neural tube morphogenesis in vitro by geometric constraints," Nature, Nature, vol. 599(7884), pages 268-272, November.
    3. Dong-Su Kim & Yong Whan Choi & Arunkumar Shanmugasundaram & Yun-Jin Jeong & Jongsung Park & Nomin-Erdene Oyunbaatar & Eung-Sam Kim & Mansoo Choi & Dong-Weon Lee, 2020. "Highly durable crack sensor integrated with silicone rubber cantilever for measuring cardiac contractility," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
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