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In situ observation of a stepwise [2 + 2] photocycloaddition process using fluorescence spectroscopy

Author

Listed:
  • Meng-Fan Wang

    (Soochow University
    Chinese Academy of Sciences)

  • Yun-Hu Deng

    (Soochow University)

  • Yu-Xuan Hong

    (Soochow University)

  • Jia-Hui Gu

    (Soochow University)

  • Yong-Yong Cao

    (Jiaxing University)

  • Qi Liu

    (Soochow University)

  • Pierre Braunstein

    (Université de Strasbourg, 4 rue Blaise Pascal - CS 90032)

  • Jian-Ping Lang

    (Soochow University
    Chinese Academy of Sciences)

Abstract

Using highly sensitive and selective in situ techniques to investigate the dynamics of intermediates formation is key to better understand reaction mechanisms. However, investigating the early stages of solid-state reactions/transformations is still challenging. Here we introduce in situ fluorescence spectroscopy to observe the evolution of intermediates during a two-step [2 + 2] photocycloaddition process in a coordination polymer platform. The structural changes and kinetics of each step under ultraviolet light irradiation versus time are accompanied by the gradual increase-decrease of intensity and blue-shift of the fluorescence spectra from the crystals. Monitoring the fluorescence behavior using a laser scanning confocal microscope can directly visualize the inhomogeneity of the photocycloaddition reaction in a single crystal. Theoretical calculations allow us to rationalize the fluorescence behavior of these compounds. We provide a convenient strategy for visualizing the solid-state photocycloaddition dynamics using fluorescence spectroscopy and open an avenue for kinetic studies of a variety of fast reactions.

Suggested Citation

  • Meng-Fan Wang & Yun-Hu Deng & Yu-Xuan Hong & Jia-Hui Gu & Yong-Yong Cao & Qi Liu & Pierre Braunstein & Jian-Ping Lang, 2023. "In situ observation of a stepwise [2 + 2] photocycloaddition process using fluorescence spectroscopy," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42604-9
    DOI: 10.1038/s41467-023-42604-9
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    References listed on IDEAS

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    1. Jonathan S. Trimble & Rebecca Crawshaw & Florence J. Hardy & Colin W. Levy & Murray J. B. Brown & Douglas E. Fuerst & Derren J. Heyes & Richard Obexer & Anthony P. Green, 2022. "A designed photoenzyme for enantioselective [2+2] cycloadditions," Nature, Nature, vol. 611(7937), pages 709-714, November.
    2. Ningning Sun & Jianjian Huang & Junyi Qian & Tai-Ping Zhou & Juan Guo & Langyu Tang & Wentao Zhang & Yaming Deng & Weining Zhao & Guojiao Wu & Rong-Zhen Liao & Xi Chen & Fangrui Zhong & Yuzhou Wu, 2022. "Enantioselective [2+2]-cycloadditions with triplet photoenzymes," Nature, Nature, vol. 611(7937), pages 715-720, November.
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