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Shadow imaging for panoptical visualization of brain tissue in vivo

Author

Listed:
  • Yulia Dembitskaya

    (CNRS UMR 5297 and University of Bordeaux)

  • Andrew K. J. Boyce

    (CNRS UMR 5297 and University of Bordeaux
    University of Calgary
    University of Calgary)

  • Agata Idziak

    (CNRS UMR 5297 and University of Bordeaux)

  • Atefeh Pourkhalili Langeroudi

    (CNRS UMR 5297 and University of Bordeaux)

  • Misa Arizono

    (CNRS UMR 5297 and University of Bordeaux
    Kyoto University Graduate School of Medicine/The Hakubi Center for Advanced Research, Kyoto University)

  • Jordan Girard

    (CNRS UMR 5297 and University of Bordeaux)

  • Guillaume Bourdellès

    (CNRS UMR 5297 and University of Bordeaux)

  • Mathieu Ducros

    (Université de Bordeaux, CNRS, INSERM, Bordeaux Imaging Center (BIC), UAR 3420, US 4)

  • Marie Sato-Fitoussi

    (CNRS UMR 5297 and University of Bordeaux)

  • Amaia Ochoa de Amezaga

    (CNRS UMR 5297 and University of Bordeaux)

  • Kristell Oizel

    (Université de Bordeaux, INSERM, Bordeaux Institute of Oncology (BRIC), U1312, Bat B2, Allée Geoffroy St Hilaire)

  • Stephane Bancelin

    (CNRS UMR 5297 and University of Bordeaux)

  • Luc Mercier

    (CNRS UMR 5297 and University of Bordeaux)

  • Thomas Pfeiffer

    (CNRS UMR 5297 and University of Bordeaux)

  • Roger J. Thompson

    (University of Calgary
    University of Calgary)

  • Sun Kwang Kim

    (CNRS UMR 5297 and University of Bordeaux
    Kyung Hee University)

  • Andreas Bikfalvi

    (Université de Bordeaux, INSERM, Bordeaux Institute of Oncology (BRIC), U1312, Bat B2, Allée Geoffroy St Hilaire)

  • U. Valentin Nägerl

    (CNRS UMR 5297 and University of Bordeaux)

Abstract

Progress in neuroscience research hinges on technical advances in visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require tissue fixation (electron microscopy), do not have cellular resolution (magnetic resonance imaging) or only give a fragmented view (fluorescence microscopy). Here, we show how regular light microscopy together with fluorescence labeling of the interstitial fluid in the extracellular space provide comprehensive optical access in real-time to the anatomical complexity and dynamics of living brain tissue at submicron scale. Using several common fluorescence microscopy modalities (confocal, light-sheet and 2-photon microscopy) in mouse organotypic and acute brain slices and the intact mouse brain in vivo, we demonstrate the value of this straightforward ‘shadow imaging’ approach by revealing neurons, microglia, tumor cells and blood capillaries together with their complete anatomical tissue contexts. In addition, we provide quantifications of perivascular spaces and the volume fraction of the extracellular space of brain tissue in vivo.

Suggested Citation

  • Yulia Dembitskaya & Andrew K. J. Boyce & Agata Idziak & Atefeh Pourkhalili Langeroudi & Misa Arizono & Jordan Girard & Guillaume Bourdellès & Mathieu Ducros & Marie Sato-Fitoussi & Amaia Ochoa de Amez, 2023. "Shadow imaging for panoptical visualization of brain tissue in vivo," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42055-2
    DOI: 10.1038/s41467-023-42055-2
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    References listed on IDEAS

    as
    1. Thomas Daubon & Céline Léon & Kim Clarke & Laetitia Andrique & Laura Salabert & Elodie Darbo & Raphael Pineau & Sylvaine Guérit & Marlène Maitre & Stéphane Dedieu & Albin Jeanne & Sabine Bailly & Jean, 2019. "Deciphering the complex role of thrombospondin-1 in glioblastoma development," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    2. Yi Chen & Patricia Pais-Roldan & Xuming Chen & Michael H. Frosz & Xin Yu, 2019. "MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
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