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MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording

Author

Listed:
  • Yi Chen

    (Max Planck Institute for Biological Cybernetics
    University of Tuebingen)

  • Patricia Pais-Roldan

    (Max Planck Institute for Biological Cybernetics
    University of Tuebingen)

  • Xuming Chen

    (Max Planck Institute for Biological Cybernetics
    Wuhan University)

  • Michael H. Frosz

    (Max Planck Institute for the Science of Light)

  • Xin Yu

    (Max Planck Institute for Biological Cybernetics
    Massachusetts General Hospital and Harvard Medical School)

Abstract

Optical fiber-mediated optogenetic activation and neuronal Ca2+ recording in combination with fMRI provide a multi-modal fMRI platform. Here, we developed an MRI-guided robotic arm (MgRA) as a flexible positioning system with high precision to real-time assist optical fiber brain intervention for multi-modal animal fMRI. Besides the ex vivo precision evaluation, we present the highly reliable brain activity patterns in the projected basal forebrain regions upon MgRA-driven optogenetic stimulation in the lateral hypothalamus. Also, we show the step-wise optical fiber targeting thalamic nuclei and map the region-specific functional connectivity with whole-brain fMRI accompanied by simultaneous calcium recordings to specify its circuit-specificity. The MgRA also guides the real-time microinjection to specific deep brain nuclei, which is demonstrated by an Mn-enhanced MRI method. The MgRA represents a clear advantage over the standard stereotaxic-based fiber implantation and opens a broad avenue to investigate the circuit-specific functional brain mapping with the multi-modal fMRI platform.

Suggested Citation

  • Yi Chen & Patricia Pais-Roldan & Xuming Chen & Michael H. Frosz & Xin Yu, 2019. "MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10450-3
    DOI: 10.1038/s41467-019-10450-3
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    Cited by:

    1. Yulia Dembitskaya & Andrew K. J. Boyce & Agata Idziak & Atefeh Pourkhalili Langeroudi & Misa Arizono & Jordan Girard & Guillaume Bourdellès & Mathieu Ducros & Marie Sato-Fitoussi & Amaia Ochoa de Amez, 2023. "Shadow imaging for panoptical visualization of brain tissue in vivo," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Vinod Menon & Domenic Cerri & Byeongwook Lee & Rui Yuan & Sung-Ho Lee & Yen-Yu Ian Shih, 2023. "Optogenetic stimulation of anterior insular cortex neurons in male rats reveals causal mechanisms underlying suppression of the default mode network by the salience network," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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