IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-41347-x.html
   My bibliography  Save this article

Peroxisomal compartmentalization of amino acid biosynthesis reactions imposes an upper limit on compartment size

Author

Listed:
  • Ying Gu

    (The Francis Crick Institute
    King’s College London)

  • Sara Alam

    (The Francis Crick Institute
    King’s College London
    Medical Research Council London Institute of Medical Sciences)

  • Snezhana Oliferenko

    (The Francis Crick Institute
    King’s College London)

Abstract

Cellular metabolism relies on just a few redox cofactors. Selective compartmentalization may prevent competition between metabolic reactions requiring the same cofactor. Is such compartmentalization necessary for optimal cell function? Is there an optimal compartment size? Here we probe these fundamental questions using peroxisomal compartmentalization of the last steps of lysine and histidine biosynthesis in the fission yeast Schizosaccharomyces japonicus. We show that compartmentalization of these NAD+ dependent reactions together with a dedicated NADH/NAD+ recycling enzyme supports optimal growth when an increased demand for anabolic reactions taxes cellular redox balance. In turn, compartmentalization constrains the size of individual organelles, with larger peroxisomes accumulating all the required enzymes but unable to support both biosynthetic reactions at the same time. Our reengineering and physiological experiments indicate that compartmentalized biosynthetic reactions are sensitive to the size of the compartment, likely due to scaling-dependent changes within the system, such as enzyme packing density.

Suggested Citation

  • Ying Gu & Sara Alam & Snezhana Oliferenko, 2023. "Peroxisomal compartmentalization of amino acid biosynthesis reactions imposes an upper limit on compartment size," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41347-x
    DOI: 10.1038/s41467-023-41347-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-41347-x
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-41347-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Mohammad Tauqeer Alam & Viridiana Olin-Sandoval & Anna Stincone & Markus A. Keller & Aleksej Zelezniak & Ben F. Luisi & Markus Ralser, 2017. "The self-inhibitory nature of metabolic networks and its alleviation through compartmentalization," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    2. Erez Persi & Miquel Duran-Frigola & Mehdi Damaghi & William R. Roush & Patrick Aloy & John L. Cleveland & Robert J. Gillies & Eytan Ruppin, 2018. "Systems analysis of intracellular pH vulnerabilities for cancer therapy," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    3. Ying Gu & Snezhana Oliferenko, 2019. "Cellular geometry scaling ensures robust division site positioning," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Clément Adam & Léa Paolini & Naïg Gueguen & Guillaume Mabilleau & Laurence Preisser & Simon Blanchard & Pascale Pignon & Florence Manero & Morgane Mao & Alain Morel & Pascal Reynier & Céline Beauvilla, 2021. "Acetoacetate protects macrophages from lactic acidosis-induced mitochondrial dysfunction by metabolic reprograming," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41347-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.