IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-40046-x.html
   My bibliography  Save this article

Deep-profiling of phospholipidome via rapid orthogonal separations and isomer-resolved mass spectrometry

Author

Listed:
  • Tian Xia

    (Tsinghua University)

  • Feng Zhou

    (Bytedance Technology Co.)

  • Donghui Zhang

    (Tsinghua University, Department of Precision Instrument)

  • Xue Jin

    (Tsinghua University)

  • Hengxue Shi

    (Tsinghua University)

  • Hang Yin

    (Tsinghua University
    Tsinghua University
    Tsinghua University)

  • Yanqing Gong

    (Peking University First Hospital)

  • Yu Xia

    (Tsinghua University)

Abstract

A lipidome comprises thousands of lipid species, many of which are isomers and isobars. Liquid chromatography-tandem mass spectrometry (LC-MS/MS), although widely used for lipidomic profiling, faces challenges in differentiating lipid isomers. Herein, we address this issue by leveraging the orthogonal separation capabilities of hydrophilic interaction liquid chromatography (HILIC) and trapped ion mobility spectrometry (TIMS). We further integrate isomer-resolved MS/MS methods onto HILIC-TIMS, which enable pinpointing double bond locations in phospholipids and sn-positions in phosphatidylcholine. This system profiles phospholipids at multiple structural levels with short analysis time (

Suggested Citation

  • Tian Xia & Feng Zhou & Donghui Zhang & Xue Jin & Hengxue Shi & Hang Yin & Yanqing Gong & Yu Xia, 2023. "Deep-profiling of phospholipidome via rapid orthogonal separations and isomer-resolved mass spectrometry," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40046-x
    DOI: 10.1038/s41467-023-40046-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-40046-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-40046-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Raissa Lerner & Dhanwin Baker & Claudia Schwitter & Sarah Neuhaus & Tony Hauptmann & Julia M. Post & Stefan Kramer & Laura Bindila, 2023. "Four-dimensional trapped ion mobility spectrometry lipidomics for high throughput clinical profiling of human blood samples," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    2. Wenpeng Zhang & Donghui Zhang & Qinhua Chen & Junhan Wu & Zheng Ouyang & Yu Xia, 2019. "Publisher Correction: Online photochemical derivatization enables comprehensive mass spectrometric analysis of unsaturated phospholipid isomers," Nature Communications, Nature, vol. 10(1), pages 1-1, December.
    3. Tongzhou Li & Yandong Yin & Zhiwei Zhou & Jiaqian Qiu & Wenbin Liu & Xueting Zhang & Kaiwen He & Yuping Cai & Zheng-Jiang Zhu, 2021. "Ion mobility-based sterolomics reveals spatially and temporally distinctive sterol lipids in the mouse brain," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    4. Denise Wolrab & Robert Jirásko & Eva Cífková & Marcus Höring & Ding Mei & Michaela Chocholoušková & Ondřej Peterka & Jakub Idkowiak & Tereza Hrnčiarová & Ladislav Kuchař & Robert Ahrends & Radana Brum, 2022. "Lipidomic profiling of human serum enables detection of pancreatic cancer," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    5. Kim Vriens & Stefan Christen & Sweta Parik & Dorien Broekaert & Kazuaki Yoshinaga & Ali Talebi & Jonas Dehairs & Carmen Escalona-Noguero & Roberta Schmieder & Thomas Cornfield & Catriona Charlton & La, 2019. "Evidence for an alternative fatty acid desaturation pathway increasing cancer plasticity," Nature, Nature, vol. 566(7744), pages 403-406, February.
    6. Wenpeng Zhang & Donghui Zhang & Qinhua Chen & Junhan Wu & Zheng Ouyang & Yu Xia, 2019. "Online photochemical derivatization enables comprehensive mass spectrometric analysis of unsaturated phospholipid isomers," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    7. Katrina L. Leaptrot & Jody C. May & James N. Dodds & John A. McLean, 2019. "Ion mobility conformational lipid atlas for high confidence lipidomics," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    8. Wenbo Cao & Simin Cheng & Jing Yang & Jiaxin Feng & Wenpeng Zhang & Zishuai Li & Qinhua Chen & Yu Xia & Zheng Ouyang & Xiaoxiao Ma, 2020. "Large-scale lipid analysis with C=C location and sn-position isomer resolving power," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Jan Philipp Menzel & Reuben S. E. Young & Aurélie H. Benfield & Julia S. Scott & Puttandon Wongsomboon & Lukáš Cudlman & Josef Cvačka & Lisa M. Butler & Sónia T. Henriques & Berwyck L. J. Poad & Steph, 2023. "Ozone-enabled fatty acid discovery reveals unexpected diversity in the human lipidome," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    2. Guifang Feng & Ming Gao & Liwei Wang & Jiayi Chen & Menglu Hou & Qiongqiong Wan & Yun Lin & Guoyong Xu & Xiaotian Qi & Suming Chen, 2022. "Dual-resolving of positional and geometric isomers of C=C bonds via bifunctional photocycloaddition-photoisomerization reaction system," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Hua Zhang & Yuan Liu & Lauren Fields & Xudong Shi & Penghsuan Huang & Haiyan Lu & Andrew J. Schneider & Xindi Tang & Luigi Puglielli & Nathan V. Welham & Lingjun Li, 2023. "Single-cell lipidomics enabled by dual-polarity ionization and ion mobility-mass spectrometry imaging," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    4. Bennett W. Fox & Maximilian J. Helf & Russell N. Burkhardt & Alexander B. Artyukhin & Brian J. Curtis & Diana Fajardo Palomino & Allen F. Schroeder & Amaresh Chaturbedi & Arnaud Tauffenberger & Cheste, 2024. "Evolutionarily related host and microbial pathways regulate fat desaturation in C. elegans," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    5. Yanying Wang & Jing Wang & Xiaoyu Li & Xushen Xiong & Jianyi Wang & Ziheng Zhou & Xiaoxiao Zhu & Yang Gu & Dan Dominissini & Lei He & Yong Tian & Chengqi Yi & Zusen Fan, 2021. "N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism," Nature Communications, Nature, vol. 12(1), pages 1-19, December.
    6. Xiaoyu Zhou & Zhuofan Wang & Jingjin Fan & Zheng Ouyang, 2023. "High-resolution separation of bioisomers using ion cloud profiling," Nature Communications, Nature, vol. 14(1), pages 1-6, December.
    7. Mingdu Luo & Yandong Yin & Zhiwei Zhou & Haosong Zhang & Xi Chen & Hongmiao Wang & Zheng-Jiang Zhu, 2023. "A mass spectrum-oriented computational method for ion mobility-resolved untargeted metabolomics," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40046-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.