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Feasibility of an implantable bioreactor for renal cell therapy using silicon nanopore membranes

Author

Listed:
  • Eun Jung Kim

    (University of California)

  • Caressa Chen

    (University of California)

  • Rebecca Gologorsky

    (University of California)

  • Ana Santandreu

    (University of California)

  • Alonso Torres

    (University of California)

  • Nathan Wright

    (University of California
    Silicon Kidney LLC)

  • Mark S. Goodin

    (SimuTech Group)

  • Jarrett Moyer

    (University of California)

  • Benjamin W. Chui

    (University of California)

  • Charles Blaha

    (University of California
    Silicon Kidney LLC)

  • Paul Brakeman

    (University of California)

  • Shant Vartanian

    (University of California)

  • Qizhi Tang

    (University of California)

  • H. David Humes

    (University of Michigan
    Innovative Biotherapies Inc)

  • William H. Fissell

    (Silicon Kidney LLC
    Vanderbilt University Medical Center)

  • Shuvo Roy

    (University of California
    Silicon Kidney LLC)

Abstract

The definitive treatment for end-stage renal disease is kidney transplantation, which remains limited by organ availability and post-transplant complications. Alternatively, an implantable bioartificial kidney could address both problems while enhancing the quality and length of patient life. An implantable bioartificial kidney requires a bioreactor containing renal cells to replicate key native cell functions, such as water and solute reabsorption, and metabolic and endocrinologic functions. Here, we report a proof-of-concept implantable bioreactor containing silicon nanopore membranes to offer a level of immunoprotection to human renal epithelial cells. After implantation into pigs without systemic anticoagulation or immunosuppression therapy for 7 days, we show that cells maintain >90% viability and functionality, with normal or elevated transporter gene expression and vitamin D activation. Despite implantation into a xenograft model, we find that cells exhibit minimal damage, and recipient cytokine levels are not suggestive of hyperacute rejection. These initial data confirm the potential feasibility of an implantable bioreactor for renal cell therapy utilizing silicon nanopore membranes.

Suggested Citation

  • Eun Jung Kim & Caressa Chen & Rebecca Gologorsky & Ana Santandreu & Alonso Torres & Nathan Wright & Mark S. Goodin & Jarrett Moyer & Benjamin W. Chui & Charles Blaha & Paul Brakeman & Shant Vartanian , 2023. "Feasibility of an implantable bioreactor for renal cell therapy using silicon nanopore membranes," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39888-2
    DOI: 10.1038/s41467-023-39888-2
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    References listed on IDEAS

    as
    1. Minoru Takasato & Pei X. Er & Han S. Chiu & Barbara Maier & Gregory J. Baillie & Charles Ferguson & Robert G. Parton & Ernst J. Wolvetang & Matthias S. Roost & Susana M. Chuva de Sousa Lopes & Melissa, 2015. "Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis," Nature, Nature, vol. 526(7574), pages 564-568, October.
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