IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-39745-2.html
   My bibliography  Save this article

Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury

Author

Listed:
  • Ao Fang

    (Zhejiang University School of Medicine
    Zhejiang University
    Zhejiang University
    Zhejiang University)

  • Yifan Wang

    (Zhejiang University School of Medicine
    Zhejiang University)

  • Naiyu Guan

    (Zhejiang University School of Medicine
    Zhejiang University
    Zhejiang University
    Zhejiang University)

  • Yanming Zuo

    (Zhejiang University School of Medicine
    Zhejiang University
    Zhejiang University)

  • Lingmin Lin

    (Zhejiang University
    Zhejiang University)

  • Binjie Guo

    (Zhejiang University
    Zhejiang University)

  • Aisheng Mo

    (Zhejiang University
    Zhejiang University)

  • Yile Wu

    (Zhejiang University
    Zhejiang University)

  • Xurong Lin

    (Zhejiang University
    Zhejiang University)

  • Wanxiong Cai

    (Zhejiang University
    Zhejiang University)

  • Xiangfeng Chen

    (Zhejiang University
    Zhejiang University
    Zhejiang University)

  • Jingjia Ye

    (Zhejiang University
    Zhejiang University
    Zhejiang University)

  • Zeinab Abdelrahman

    (Zhejiang University School of Medicine
    Zhejiang University
    Zhejiang University
    Zhejiang University)

  • Xiaodan Li

    (Zhejiang University School of Medicine
    Zhejiang University
    Zhejiang University
    Zhejiang University)

  • Hanyu Zheng

    (Zhejiang University
    Zhejiang University)

  • Zhonghan Wu

    (Zhejiang University
    Zhejiang University)

  • Shuang Jin

    (Zhejiang University
    Zhejiang University)

  • Kan Xu

    (Zhejiang University)

  • Yan Huang

    (Medical School of Nantong University)

  • Xiaosong Gu

    (Nantong University)

  • Bin Yu

    (Nantong University)

  • Xuhua Wang

    (Zhejiang University School of Medicine
    Zhejiang University
    Zhejiang University
    Zhejiang University)

Abstract

The transplantation of mesenchymal stem cells-derived secretome, particularly extracellular vesicles is a promising therapy to suppress spinal cord injury-triggered neuroinflammation. However, efficient delivery of extracellular vesicles to the injured spinal cord, with minimal damage, remains a challenge. Here we present a device for the delivery of extracellular vesicles to treat spinal cord injury. We show that the device incorporating mesenchymal stem cells and porous microneedles enables the delivery of extracellular vesicles. We demonstrate that topical application to the spinal cord lesion beneath the spinal dura, does not damage the lesion. We evaluate the efficacy of our device in a contusive spinal cord injury model and find that it reduces the cavity and scar tissue formation, promotes angiogenesis, and improves survival of nearby tissues and axons. Importantly, the sustained delivery of extracellular vesicles for at least 7 days results in significant functional recovery. Thus, our device provides an efficient and sustained extracellular vesicles delivery platform for spinal cord injury treatment.

Suggested Citation

  • Ao Fang & Yifan Wang & Naiyu Guan & Yanming Zuo & Lingmin Lin & Binjie Guo & Aisheng Mo & Yile Wu & Xurong Lin & Wanxiong Cai & Xiangfeng Chen & Jingjia Ye & Zeinab Abdelrahman & Xiaodan Li & Hanyu Zh, 2023. "Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39745-2
    DOI: 10.1038/s41467-023-39745-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-39745-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-39745-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Chi Lam Au Yeung & Ngai-Na Co & Tetsushi Tsuruga & Tsz-Lun Yeung & Suet-Ying Kwan & Cecilia S. Leung & Yong Li & Edward S. Lu & Kenny Kwan & Kwong-Kwok Wong & Rosemarie Schmandt & Karen H. Lu & Samuel, 2016. "Exosomal transfer of stroma-derived miR21 confers paclitaxel resistance in ovarian cancer cells through targeting APAF1," Nature Communications, Nature, vol. 7(1), pages 1-14, September.
    2. Benedikt Brommer & Miao He & Zicong Zhang & Zhiyun Yang & Jessica C. Page & Junfeng Su & Yu Zhang & Junjie Zhu & Emilia Gouy & Jing Tang & Philip Williams & Wei Dai & Qi Wang & Ryan Solinsky & Bo Chen, 2021. "Improving hindlimb locomotor function by Non-invasive AAV-mediated manipulations of propriospinal neurons in mice with complete spinal cord injury," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yongheng Fan & Xianming Wu & Sufang Han & Qi Zhang & Zheng Sun & Bing Chen & Xiaoyu Xue & Haipeng Zhang & Zhenni Chen & Man Yin & Zhifeng Xiao & Yannan Zhao & Jianwu Dai, 2023. "Single-cell analysis reveals region-heterogeneous responses in rhesus monkey spinal cord with complete injury," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    2. Chun-Xiao Huang & Yacong Zhao & Jie Mao & Zhen Wang & Lulu Xu & Jianwei Cheng & Na N. Guan & Jianren Song, 2021. "An injury-induced serotonergic neuron subpopulation contributes to axon regrowth and function restoration after spinal cord injury in zebrafish," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39745-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.