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Nuclear lamina strain states revealed by intermolecular force biosensor

Author

Listed:
  • Brooke E. Danielsson

    (Virginia Commonwealth University)

  • Bobin George Abraham

    (Tampere University)

  • Elina Mäntylä

    (Tampere University)

  • Jolene I. Cabe

    (Virginia Commonwealth University)

  • Carl R. Mayer

    (Virginia Commonwealth University)

  • Anna Rekonen

    (Tampere University)

  • Frans Ek

    (Tampere University)

  • Daniel E. Conway

    (The Ohio State University
    The Ohio State University)

  • Teemu O. Ihalainen

    (Tampere University
    Tampere University)

Abstract

Nuclear lamins have been considered an important structural element of the nucleus. The nuclear lamina is thought both to shield DNA from excessive mechanical forces and to transmit mechanical forces onto the DNA. However, to date there is not yet a technical approach to directly measure mechanical forces on nuclear lamins at the protein level. To overcome this limitation, we developed a nanobody-based intermolecular tension FRET biosensor capable of measuring the mechanical strain of lamin filaments. Using this sensor, we were able to show that the nuclear lamina is subjected to significant force. These forces are dependent on nuclear volume, actomyosin contractility, functional LINC complex, chromatin condensation state, cell cycle, and EMT. Interestingly, large forces were also present on nucleoplasmic lamins, indicating that these lamins may also have an important mechanical role in the nucleus. Overall, we demonstrate that the nanobody-based approach allows construction of biosensors for complex protein structures for mechanobiology studies.

Suggested Citation

  • Brooke E. Danielsson & Bobin George Abraham & Elina Mäntylä & Jolene I. Cabe & Carl R. Mayer & Anna Rekonen & Frans Ek & Daniel E. Conway & Teemu O. Ihalainen, 2023. "Nuclear lamina strain states revealed by intermolecular force biosensor," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39563-6
    DOI: 10.1038/s41467-023-39563-6
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    References listed on IDEAS

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