IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-38941-4.html
   My bibliography  Save this article

Coherent Stokes Raman scattering microscopy (CSRS)

Author

Listed:
  • Sandro Heuke

    (Institut Fresnel)

  • Hervé Rigneault

    (Institut Fresnel)

Abstract

We report the first implementation of laser scanning coherent Stokes Raman scattering (CSRS) microscopy. To overcome the major challenge in CSRS imaging, we show how to suppress the fluorescence background by narrow bandpass filter and a lock-in based demodulation. Near background free CSRS imaging of polymer beads, human skin, onion cells, avocado flesh and the wing disc of a drosphila larva are presented. Finally, we explain and demonstrate numerically that CSRS solves a major obstacle of other coherent Raman techniques by sending a significant part (up to 100%) of the CSRS photons into the backward direction under tight focusing conditions. We believe that this discovery will pave the way for numerous technological advances, e.g., in epi-detected coherent Raman multi-focus imaging, real-time laser scanning based spectroscopy or efficient endoscopy.

Suggested Citation

  • Sandro Heuke & Hervé Rigneault, 2023. "Coherent Stokes Raman scattering microscopy (CSRS)," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38941-4
    DOI: 10.1038/s41467-023-38941-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-38941-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-38941-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Lu Wei & Zhixing Chen & Lixue Shi & Rong Long & Andrew V. Anzalone & Luyuan Zhang & Fanghao Hu & Rafael Yuste & Virginia W. Cornish & Wei Min, 2017. "Super-multiplex vibrational imaging," Nature, Nature, vol. 544(7651), pages 465-470, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yiyi Zhang & Sidan Tian & Liping Huang & Yanan Li & Yuan Lu & Hongyu Li & Guiping Chen & Fanling Meng & Gang L. Liu & Xiangliang Yang & Jiasheng Tu & Chunmeng Sun & Liang Luo, 2022. "Reactive oxygen species-responsive and Raman-traceable hydrogel combining photodynamic and immune therapy for postsurgical cancer treatment," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Yueli Yang & Xueyang Bai & Fanghao Hu, 2024. "Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    3. Raja Chouket & Agnès Pellissier-Tanon & Aliénor Lahlou & Ruikang Zhang & Diana Kim & Marie-Aude Plamont & Mingshu Zhang & Xi Zhang & Pingyong Xu & Nicolas Desprat & Dominique Bourgeois & Agathe Espagn, 2022. "Extra kinetic dimensions for label discrimination," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    4. Xiujuan Gao & Xinyuan Huang & Zhongyun Chen & Liu Yang & Yifu Zhou & Zhenxuan Hou & Jie Yang & Shuhong Qi & Zheng Liu & Zhihong Zhang & Qian Liu & Qingming Luo & Ling Fu, 2024. "Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    5. Xiaomeng Han & Xiaotang Lu & Peter H. Li & Shuohong Wang & Richard Schalek & Yaron Meirovitch & Zudi Lin & Jason Adhinarta & Karl D. Murray & Leah M. MacNiven & Daniel R. Berger & Yuelong Wu & Tao Fan, 2024. "Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    6. Yingfeng Tao & Xiaoliu Zhou & Leqiang Sun & Da Lin & Huaiyuan Cai & Xi Chen & Wei Zhou & Bing Yang & Zhe Hu & Jing Yu & Jing Zhang & Xiaoqing Yang & Fang Yang & Bang Shen & Wenbao Qi & Zhenfang Fu & J, 2023. "Highly efficient and robust π-FISH rainbow for multiplexed in situ detection of diverse biomolecules," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38941-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.