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A highly sensitive strategy for monitoring real-time proliferation of targeted cell types in vivo

Author

Listed:
  • Hiroto Sugawara

    (Tohoku University Graduate School of Medicine)

  • Junta Imai

    (Tohoku University Graduate School of Medicine)

  • Junpei Yamamoto

    (Tohoku University Graduate School of Medicine)

  • Tomohito Izumi

    (Tohoku University Graduate School of Medicine)

  • Yohei Kawana

    (Tohoku University Graduate School of Medicine)

  • Akira Endo

    (Tohoku University Graduate School of Medicine)

  • Masato Kohata

    (Tohoku University Graduate School of Medicine)

  • Junro Seike

    (Tohoku University Graduate School of Medicine)

  • Haremaru Kubo

    (Tohoku University Graduate School of Medicine)

  • Hiroshi Komamura

    (Tohoku University Graduate School of Medicine)

  • Yuichiro Munakata

    (Tohoku Medical and Pharmaceutical University)

  • Yoichiro Asai

    (Tohoku University Graduate School of Medicine)

  • Shinichiro Hosaka

    (Tohoku University Graduate School of Medicine)

  • Shojiro Sawada

    (Tohoku Medical and Pharmaceutical University)

  • Shinjiro Kodama

    (Tohoku University Graduate School of Medicine)

  • Kei Takahashi

    (Tohoku University Graduate School of Medicine)

  • Keizo Kaneko

    (Tohoku University Graduate School of Medicine)

  • Hideki Katagiri

    (Tohoku University Graduate School of Medicine)

Abstract

Cell proliferation processes play pivotal roles in timely adaptation to many biological situations. Herein, we establish a highly sensitive and simple strategy by which time-series showing the proliferation of a targeted cell type can be quantitatively monitored in vivo in the same individuals. We generate mice expressing a secreted type of luciferase only in cells producing Cre under the control of the Ki67 promoter. Crossing these with tissue-specific Cre-expressing mice allows us to monitor the proliferation time course of pancreatic β-cells, which are few in number and weakly proliferative, by measuring plasma luciferase activity. Physiological time courses, during obesity development, pregnancy and juvenile growth, as well as diurnal variation, of β-cell proliferation, are clearly detected. Moreover, this strategy can be utilized for highly sensitive ex vivo screening for proliferative factors for targeted cells. Thus, these technologies may contribute to advancements in broad areas of biological and medical research.

Suggested Citation

  • Hiroto Sugawara & Junta Imai & Junpei Yamamoto & Tomohito Izumi & Yohei Kawana & Akira Endo & Masato Kohata & Junro Seike & Haremaru Kubo & Hiroshi Komamura & Yuichiro Munakata & Yoichiro Asai & Shini, 2023. "A highly sensitive strategy for monitoring real-time proliferation of targeted cell types in vivo," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38897-5
    DOI: 10.1038/s41467-023-38897-5
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    References listed on IDEAS

    as
    1. Yuval Dor & Juliana Brown & Olga I. Martinez & Douglas A. Melton, 2004. "Adult pancreatic β-cells are formed by self-duplication rather than stem-cell differentiation," Nature, Nature, vol. 429(6987), pages 41-46, May.
    2. Tomohito Izumi & Junta Imai & Junpei Yamamoto & Yohei Kawana & Akira Endo & Hiroto Sugawara & Masato Kohata & Yoichiro Asai & Kei Takahashi & Shinjiro Kodama & Keizo Kaneko & Junhong Gao & Kenji Uno &, 2018. "Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
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