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A smart pathogen detector engineered from intracellular hydrogelation of DNA-decorated macrophages

Author

Listed:
  • Yueyue Gui

    (Hefei University of Technology)

  • Yujing Zeng

    (Nanjing University)

  • Binrui Chen

    (Hefei University of Technology)

  • Yueping Yang

    (Hefei University of Technology)

  • Jiehua Ma

    (Tongren Hospital, Shanghai Jiao Tong University School of Medicine)

  • Chao Li

    (Hefei University of Technology)

Abstract

Bacterial infection is a major threat to global public health, which urgently requires useful tools to rapidly analyze pathogens in the early stages of infection. Herein, we develop a smart macrophage (Mø)-based bacteria detector, which can recognize, capture, enrich and detect different bacteria and their secreted exotoxins. We transform the fragile native Møs into robust gelated cell particles (GMøs) using photo-activated crosslinking chemistry, which retains membrane integrity and recognition capacity for different microbes. Meanwhile, these GMøs equipped with magnetic nanoparticles and DNA sensing elements can not only respond to an external magnet for facile bacteria collection, but allow the detection of multiple types of bacteria in a single assay. Additionally, we design a propidium iodide-based staining assay to rapidly detect pathogen-associated exotoxins at ultralow concentrations. Overall, these nanoengineered cell particles have broad applicability in the analysis of bacteria, and could potentially be used for the management and diagnosis of infectious diseases.

Suggested Citation

  • Yueyue Gui & Yujing Zeng & Binrui Chen & Yueping Yang & Jiehua Ma & Chao Li, 2023. "A smart pathogen detector engineered from intracellular hydrogelation of DNA-decorated macrophages," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38733-w
    DOI: 10.1038/s41467-023-38733-w
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    References listed on IDEAS

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    1. Alan Aderem & Richard J. Ulevitch, 2000. "Toll-like receptors in the induction of the innate immune response," Nature, Nature, vol. 406(6797), pages 782-787, August.
    2. Jung-Chen Lin & Chen-Ying Chien & Chi-Long Lin & Bing-Yu Yao & Yuan-I Chen & Yu-Han Liu & Zih-Syun Fang & Jui-Yi Chen & Wei-ya Chen & No-No Lee & Hui-Wen Chen & Che-Ming J. Hu, 2019. "Intracellular hydrogelation preserves fluid and functional cell membrane interfaces for biological interactions," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
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