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Hydrogenase-based oxidative biocatalysis without oxygen

Author

Listed:
  • Ammar Al-Shameri

    (Technical University of Munich)

  • Dominik L. Siebert

    (Technical University of Munich)

  • Samuel Sutiono

    (Technical University of Munich)

  • Lars Lauterbach

    (RWTH Universität Aachen, Institute of Applied Microbiology)

  • Volker Sieber

    (Technical University of Munich
    Technical University of Munich
    Technical University of Munich
    The University of Queensland)

Abstract

Biocatalysis-based synthesis can provide a sustainable and clean platform for producing chemicals. Many oxidative biocatalytic routes require the cofactor NAD+ as an electron acceptor. To date, NADH oxidase (NOX) remains the most widely applied system for NAD+ regeneration. However, its dependence on O2 implies various technical challenges in terms of O2 supply, solubility, and mass transfer. Here, we present the suitability of a NAD+ regeneration system in vitro based on H2 evolution. The efficiency of the hydrogenase-based system is demonstrated by integrating it into a multi-enzymatic cascade to produce ketoacids from sugars. The total NAD+ recycled using the hydrogenase system outperforms NOX in all different setups reaching up to 44,000 mol per mol enzyme. This system proves to be scalable and superior to NOX in terms of technical simplicity, flexibility, and total output. Furthermore, the system produces only green H2 as a by-product even in the presence of O2.

Suggested Citation

  • Ammar Al-Shameri & Dominik L. Siebert & Samuel Sutiono & Lars Lauterbach & Volker Sieber, 2023. "Hydrogenase-based oxidative biocatalysis without oxygen," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38227-9
    DOI: 10.1038/s41467-023-38227-9
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    Cited by:

    1. Pritam Giri & Seonga Lim & Taresh P. Khobragade & Amol D. Pagar & Mahesh D. Patil & Sharad Sarak & Hyunwoo Jeon & Sangwoo Joo & Younghwan Goh & Seohee Jung & Yu-Jeong Jang & Seung Beom Choi & Ye Chan , 2024. "Biocatalysis enables the scalable conversion of biobased furans into various furfurylamines," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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