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Chemical imaging reveals diverse functions of tricarboxylic acid metabolites in root growth and development

Author

Listed:
  • Tao Zhang

    (University of California San Diego)

  • Sarah E. Noll

    (Stanford University
    Pomona College)

  • Jesus T. Peng

    (University of California San Diego)

  • Amman Klair

    (University of California San Diego)

  • Abigail Tripka

    (University of California San Diego)

  • Nathan Stutzman

    (University of California San Diego)

  • Casey Cheng

    (University of California San Diego)

  • Richard N. Zare

    (Stanford University)

  • Alexandra J. Dickinson

    (University of California San Diego)

Abstract

Understanding how plants grow is critical for agriculture and fundamental for illuminating principles of multicellular development. Here, we apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to the chemical mapping of the developing maize root. This technique reveals a range of small molecule distribution patterns across the gradient of stem cell differentiation in the root. To understand the developmental logic of these patterns, we examine tricarboxylic acid (TCA) cycle metabolites. In both Arabidopsis and maize, we find evidence that elements of the TCA cycle are enriched in developmentally opposing regions. We find that these metabolites, particularly succinate, aconitate, citrate, and α-ketoglutarate, control root development in diverse and distinct ways. Critically, the developmental effects of certain TCA metabolites on stem cell behavior do not correlate with changes in ATP production. These results present insights into development and suggest practical means for controlling plant growth.

Suggested Citation

  • Tao Zhang & Sarah E. Noll & Jesus T. Peng & Amman Klair & Abigail Tripka & Nathan Stutzman & Casey Cheng & Richard N. Zare & Alexandra J. Dickinson, 2023. "Chemical imaging reveals diverse functions of tricarboxylic acid metabolites in root growth and development," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38150-z
    DOI: 10.1038/s41467-023-38150-z
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    References listed on IDEAS

    as
    1. Bryce W. Carey & Lydia W. S. Finley & Justin R. Cross & C. David Allis & Craig B. Thompson, 2015. "Intracellular α-ketoglutarate maintains the pluripotency of embryonic stem cells," Nature, Nature, vol. 518(7539), pages 413-416, February.
    2. Inmaculada Martínez-Reyes & Navdeep S. Chandel, 2020. "Mitochondrial TCA cycle metabolites control physiology and disease," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    3. Masashi Yamada & Xinwei Han & Philip N. Benfey, 2020. "RGF1 controls root meristem size through ROS signalling," Nature, Nature, vol. 577(7788), pages 85-88, January.
    Full references (including those not matched with items on IDEAS)

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    1. Zhicheng Jiang & Zihao Wei & Jun Zhang & Chenxing Zheng & Hong Zhu & Hong Zhai & Shaozhen He & Shaopei Gao & Ning Zhao & Huan Zhang & Qingchang Liu, 2024. "Source-sink synergy is the key unlocking sweet potato starch yield potential," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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