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Single-cell analysis of peripheral blood from high-altitude pulmonary hypertension patients identifies a distinct monocyte phenotype

Author

Listed:
  • Xin-Hua Wu

    (The First Affiliated Hospital of Dali University)

  • Yang-Yang He

    (Henan University)

  • Zhang-Rong Chen

    (The First Affiliated Hospital of Dali University
    The Affiliated Hospital of Guizhou Medical University)

  • Ze-Yuan He

    (Yulong People’s Hospital)

  • Yi Yan

    (Shanghai Jiao Tong University School of Medicine)

  • Yangzhige He

    (Chinese Academy of Medical Sciences & Peking Union Medical College)

  • Guang-Ming Wang

    (The First Affiliated Hospital of Dali University)

  • Yu Dong

    (The First Affiliated Hospital of Dali University)

  • Ying Yang

    (The First Affiliated Hospital of Dali University)

  • Yi-Min Sun

    (CapitalBio Technology Corporation)

  • Yong-Hong Ren

    (CapitalBio Technology Corporation)

  • Qiu-Yan Zhao

    (The First Affiliated Hospital of Dali University)

  • Xiao-Dan Yang

    (The First Affiliated Hospital of Dali University)

  • Li-Ying Wang

    (The First Affiliated Hospital of Dali University)

  • Cai-Jun Fu

    (The First Affiliated Hospital of Dali University)

  • Miao He

    (Dali University)

  • Si-Jin Zhang

    (Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine)

  • Ji-Fen Fu

    (The First Affiliated Hospital of Dali University)

  • Hong Liu

    (The First Affiliated Hospital of Dali University)

  • Zhi-Cheng Jing

    (Chinese Academy of Medical Sciences & Peking Union Medical College)

Abstract

Immune and inflammatory responses have an important function in the pathophysiology of pulmonary hypertension (PH). However, little is known about the immune landscape in peripheral circulation in patients with high-altitude pulmonary hypertension (HAPH). We apply single-cell transcriptomics to characterize the monocytes that are significantly enriched in the peripheral blood mononuclear cells (PBMC) of HAPH patients. We discover an increase in C1 (non-classical) and C2 (intermediate) monocytes in PBMCs and a decrease in hypoxia-inducible transcription factor-1α (HIF-1α) in all monocyte subsets associated with HAPH. In addition, we demonstrate that similar immune adaptations may exist in HAPH and PH. Overall, we characterize an immune cell atlas of the peripheral blood in HAPH patients. Our data provide evidence that specific monocyte subsets and HIF-1α downregulation might be implicated in the pathogenesis of HAPH.

Suggested Citation

  • Xin-Hua Wu & Yang-Yang He & Zhang-Rong Chen & Ze-Yuan He & Yi Yan & Yangzhige He & Guang-Ming Wang & Yu Dong & Ying Yang & Yi-Min Sun & Yong-Hong Ren & Qiu-Yan Zhao & Xiao-Dan Yang & Li-Ying Wang & Ca, 2023. "Single-cell analysis of peripheral blood from high-altitude pulmonary hypertension patients identifies a distinct monocyte phenotype," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37527-4
    DOI: 10.1038/s41467-023-37527-4
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    References listed on IDEAS

    as
    1. Monika Graf & Marta Interlandi & Natalia Moreno & Dörthe Holdhof & Carolin Göbel & Viktoria Melcher & Julius Mertins & Thomas K. Albert & Dennis Kastrati & Amelie Alfert & Till Holsten & Flavia de Far, 2022. "Single-cell transcriptomics identifies potential cells of origin of MYC rhabdoid tumors," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    2. Rahul Kumar & Claudia Mickael & Biruk Kassa & Liya Gebreab & Jeffrey C. Robinson & Daniel E. Koyanagi & Linda Sanders & Lea Barthel & Christina Meadows & Daniel Fox & David Irwin & Min Li & B. Alexand, 2017. "TGF-β activation by bone marrow-derived thrombospondin-1 causes Schistosoma- and hypoxia-induced pulmonary hypertension," Nature Communications, Nature, vol. 8(1), pages 1-13, August.
    3. Yongting Luo & Xiao Teng & Lingling Zhang & Jianan Chen & Zheng Liu & Xuehui Chen & Shuai Zhao & Sai Yang & Jing Feng & Xiyun Yan, 2019. "CD146-HIF-1α hypoxic reprogramming drives vascular remodeling and pulmonary arterial hypertension," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    4. Yongting Luo & Xiao Teng & Lingling Zhang & Jianan Chen & Zheng Liu & Xuehui Chen & Shuai Zhao & Sai Yang & Jing Feng & Xiyun Yan, 2019. "Publisher Correction: CD146-HIF-1α hypoxic reprogramming drives vascular remodeling and pulmonary arterial hypertension," Nature Communications, Nature, vol. 10(1), pages 1-1, December.
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