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The Trypanosoma cruzi Antigen and Epitope Atlas: antibody specificities in Chagas disease patients across the Americas

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  • Alejandro D. Ricci

    (Instituto de Investigaciones Biotecnológicas (IIB) — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad de San Martín (UNSAM))

  • Leonel Bracco

    (Instituto de Investigaciones Biotecnológicas (IIB) — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad de San Martín (UNSAM))

  • Emir Salas-Sarduy

    (Instituto de Investigaciones Biotecnológicas (IIB) — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad de San Martín (UNSAM))

  • Janine M. Ramsey

    (Instituto Nacional de Salud Pública)

  • Melissa S. Nolan

    (University of South Carolina)

  • M. Katie Lynn

    (University of South Carolina)

  • Jaime Altcheh

    (Ciudad Autónoma de Buenos Aires
    Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas (IMIPP) — GCBA-CONICET)

  • Griselda E. Ballering

    (Ciudad Autónoma de Buenos Aires)

  • Faustino Torrico

    (Fundación CEADES)

  • Norival Kesper

    (Universidade de São Paulo)

  • Juan C. Villar

    (Universidad Autónoma de Bucaramanga y Fundación Cardioinfantil — Instituto de Cardiología)

  • Iván S. Marcipar

    (Universidad Nacional del Litoral)

  • Jorge D. Marco

    (Universidad Nacional de Salta — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET))

  • Fernán Agüero

    (Instituto de Investigaciones Biotecnológicas (IIB) — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad de San Martín (UNSAM))

Abstract

During an infection the immune system produces pathogen-specific antibodies. These antibody repertoires become specific to the history of infections and represent a rich source of diagnostic markers. However, the specificities of these antibodies are mostly unknown. Here, using high-density peptide arrays we examined the human antibody repertoires of Chagas disease patients. Chagas disease is a neglected disease caused by Trypanosoma cruzi, a protozoan parasite that evades immune mediated elimination and mounts long-lasting chronic infections. We describe a proteome-wide search for antigens, characterised their linear epitopes, and show their reactivity on 71 individuals from diverse human populations. Using single-residue mutagenesis we revealed the core functional residues for 232 of these epitopes. Finally, we show the diagnostic performance of identified antigens on challenging samples. These datasets enable the study of the Chagas antibody repertoire at an unprecedented depth and granularity, while also providing a rich source of serological biomarkers.

Suggested Citation

  • Alejandro D. Ricci & Leonel Bracco & Emir Salas-Sarduy & Janine M. Ramsey & Melissa S. Nolan & M. Katie Lynn & Jaime Altcheh & Griselda E. Ballering & Faustino Torrico & Norival Kesper & Juan C. Villa, 2023. "The Trypanosoma cruzi Antigen and Epitope Atlas: antibody specificities in Chagas disease patients across the Americas," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37522-9
    DOI: 10.1038/s41467-023-37522-9
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    References listed on IDEAS

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    1. Joseph Barten Legutki & Zhan-Gong Zhao & Matt Greving & Neal Woodbury & Stephen Albert Johnston & Phillip Stafford, 2014. "Scalable high-density peptide arrays for comprehensive health monitoring," Nature Communications, Nature, vol. 5(1), pages 1-7, December.
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