IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-37377-0.html
   My bibliography  Save this article

Single cell transcriptomic analysis of HPV16-infected epithelium identifies a keratinocyte subpopulation implicated in cancer

Author

Listed:
  • Mary C. Bedard

    (Cincinnati Children’s Hospital Medical Center)

  • Tafadzwa Chihanga

    (Cincinnati Children’s Hospital Medical Center)

  • Adrean Carlile

    (Cincinnati Children’s Hospital Medical Center)

  • Robert Jackson

    (University of Arizona)

  • Marion G. Brusadelli

    (Division of Clinical Operations, Medpace)

  • Denis Lee

    (University of Wisconsin)

  • Andrew VonHandorf

    (Cincinnati Children’s Hospital Medical Center)

  • Mark Rochman

    (Cincinnati Children’s Hospital Medical Center)

  • Phillip J. Dexheimer

    (Cincinnati Children’s Hospital Medical Center)

  • Jeffrey Chalmers

    (William G. Lowrie Department of Chemical and Biomolecular Engineering, Ohio State University)

  • Gerard Nuovo

    (Ohio State University Medical Center)

  • Maria Lehn

    (University of Cincinnati College of Medicine)

  • David E. J. Williams

    (University of Arizona
    University of Arizona)

  • Aditi Kulkarni

    (University of Pittsburgh
    UPMC Hillman Cancer Center)

  • Molly Carey

    (University of Cincinnati Medical Center)

  • Amanda Jackson

    (University of Cincinnati Medical Center)

  • Caroline Billingsley

    (University of Cincinnati Medical Center)

  • Alice Tang

    (University of Cincinnati)

  • Chad Zender

    (University of Cincinnati)

  • Yash Patil

    (University of Cincinnati)

  • Trisha M. Wise-Draper

    (University of Cincinnati College of Medicine)

  • Thomas J. Herzog

    (University of Cincinnati Medical Center)

  • Robert L. Ferris

    (University of Pittsburgh
    UPMC Hillman Cancer Center
    University of Pittsburgh
    University of Pittsburgh)

  • Ady Kendler

    (University of Cincinnati College of Medicine)

  • Bruce J. Aronow

    (Cincinnati Children’s Hospital Medical Center
    Cincinnati Children’s Hospital Medical Center
    University of Cincinnati College of Medicine)

  • Matthew Kofron

    (Cincinnati Children’s Hospital Medical Center
    University of Cincinnati College of Medicine)

  • Marc E. Rothenberg

    (Cincinnati Children’s Hospital Medical Center
    University of Cincinnati College of Medicine)

  • Matthew T. Weirauch

    (Cincinnati Children’s Hospital Medical Center
    University of Cincinnati College of Medicine
    Cincinnati Children’s Hospital Medical Center)

  • Koenraad Doorslaer

    (University of Arizona
    University of Arizona
    The BIO5 Institute, University of Arizona
    University of Arizona)

  • Kathryn A. Wikenheiser-Brokamp

    (University of Cincinnati College of Medicine
    Cincinnati Children’s Hospital Medical Center)

  • Paul F. Lambert

    (University of Wisconsin)

  • Mike Adam

    (Cincinnati Children’s Hospital Medical Center)

  • S. Steven Potter

    (Cincinnati Children’s Hospital Medical Center)

  • Susanne I. Wells

    (Cincinnati Children’s Hospital Medical Center
    University of Cincinnati College of Medicine)

Abstract

Persistent HPV16 infection is a major cause of the global cancer burden. The viral life cycle is dependent on the differentiation program of stratified squamous epithelium, but the landscape of keratinocyte subpopulations which support distinct phases of the viral life cycle has yet to be elucidated. Here, single cell RNA sequencing of HPV16 infected compared to uninfected organoids identifies twelve distinct keratinocyte populations, with a subset mapped to reconstruct their respective 3D geography in stratified squamous epithelium. Instead of conventional terminally differentiated cells, an HPV-reprogrammed keratinocyte subpopulation (HIDDEN cells) forms the surface compartment and requires overexpression of the ELF3/ESE-1 transcription factor. HIDDEN cells are detected throughout stages of human carcinogenesis including primary human cervical intraepithelial neoplasias and HPV positive head and neck cancers, and a possible role in promoting viral carcinogenesis is supported by TCGA analyses. Single cell transcriptome information on HPV-infected versus uninfected epithelium will enable broader studies of the role of individual keratinocyte subpopulations in tumor virus infection and cancer evolution.

Suggested Citation

  • Mary C. Bedard & Tafadzwa Chihanga & Adrean Carlile & Robert Jackson & Marion G. Brusadelli & Denis Lee & Andrew VonHandorf & Mark Rochman & Phillip J. Dexheimer & Jeffrey Chalmers & Gerard Nuovo & Ma, 2023. "Single cell transcriptomic analysis of HPV16-infected epithelium identifies a keratinocyte subpopulation implicated in cancer," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37377-0
    DOI: 10.1038/s41467-023-37377-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-37377-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-37377-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Zdenek Andrysik & Heather Bender & Matthew D. Galbraith & Joaquin M. Espinosa, 2021. "Multi-omics analysis reveals contextual tumor suppressive and oncogenic gene modules within the acute hypoxic response," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Shaun Scaramuzza & Rebecca M. Jones & Martina Muste Sadurni & Alicja Reynolds-Winczura & Divyasree Poovathumkadavil & Abigail Farrell & Toyoaki Natsume & Patricia Rojas & Cyntia Fernandez Cuesta & Mas, 2023. "TRAIP resolves DNA replication-transcription conflicts during the S-phase of unperturbed cells," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37377-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.