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Arabidopsis TRB proteins function in H3K4me3 demethylation by recruiting JMJ14

Author

Listed:
  • Ming Wang

    (University of California at Los Angeles)

  • Zhenhui Zhong

    (University of California at Los Angeles)

  • Javier Gallego-Bartolomé

    (University of California at Los Angeles
    CSIC-Universitat Politècnica de València)

  • Suhua Feng

    (University of California at Los Angeles
    University of California at Los Angeles)

  • Yuan-Hsin Shih

    (University of California at Los Angeles
    National Taiwan University)

  • Mukun Liu

    (University of California at Los Angeles)

  • Jessica Zhou

    (University of California at Los Angeles)

  • John Curtis Richey

    (University of California at Los Angeles)

  • Charmaine Ng

    (University of California at Los Angeles)

  • Yasaman Jami-Alahmadi

    (University of California at Los Angeles)

  • James Wohlschlegel

    (University of California at Los Angeles)

  • Keqiang Wu

    (National Taiwan University)

  • Steven E. Jacobsen

    (University of California at Los Angeles
    University of California at Los Angeles
    University of California at Los Angeles
    University of California at Los Angeles)

Abstract

Arabidopsis telomeric repeat binding factors (TRBs) can bind telomeric DNA sequences to protect telomeres from degradation. TRBs can also recruit Polycomb Repressive Complex 2 (PRC2) to deposit tri-methylation of H3 lysine 27 (H3K27me3) over certain target loci. Here, we demonstrate that TRBs also associate and colocalize with JUMONJI14 (JMJ14) and trigger H3K4me3 demethylation at some loci. The trb1/2/3 triple mutant and the jmj14-1 mutant show an increased level of H3K4me3 over TRB and JMJ14 binding sites, resulting in up-regulation of their target genes. Furthermore, tethering TRBs to the promoter region of genes with an artificial zinc finger (TRB-ZF) successfully triggers target gene silencing, as well as H3K27me3 deposition, and H3K4me3 removal. Interestingly, JMJ14 is predominantly recruited to ZF off-target sites with low levels of H3K4me3, which is accompanied with TRB-ZFs triggered H3K4me3 removal at these loci. These results suggest that TRB proteins coordinate PRC2 and JMJ14 activities to repress target genes via H3K27me3 deposition and H3K4me3 removal.

Suggested Citation

  • Ming Wang & Zhenhui Zhong & Javier Gallego-Bartolomé & Suhua Feng & Yuan-Hsin Shih & Mukun Liu & Jessica Zhou & John Curtis Richey & Charmaine Ng & Yasaman Jami-Alahmadi & James Wohlschlegel & Keqiang, 2023. "Arabidopsis TRB proteins function in H3K4me3 demethylation by recruiting JMJ14," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37263-9
    DOI: 10.1038/s41467-023-37263-9
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    References listed on IDEAS

    as
    1. Wanlu Liu & Javier Gallego-Bartolomé & Yuxing Zhou & Zhenhui Zhong & Ming Wang & Somsakul Pop Wongpalee & Jason Gardiner & Suhua Feng & Peggy Hsuanyu Kuo & Steven E. Jacobsen, 2021. "Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Shuiming Qian & Xinchen Lv & Ray N. Scheid & Li Lu & Zhenlin Yang & Wei Chen & Rui Liu & Melissa D. Boersma & John M. Denu & Xuehua Zhong & Jiamu Du, 2018. "Dual recognition of H3K4me3 and H3K27me3 by a plant histone reader SHL," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
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