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Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase

Author

Listed:
  • Wanlu Liu

    (Zhejiang University
    Zhejiang University
    University of California at Los Angeles)

  • Javier Gallego-Bartolomé

    (University of California at Los Angeles
    CSIC-Universidad Politécnica de Valencia)

  • Yuxing Zhou

    (Zhejiang University)

  • Zhenhui Zhong

    (University of California at Los Angeles)

  • Ming Wang

    (University of California at Los Angeles)

  • Somsakul Pop Wongpalee

    (University of California at Los Angeles
    Faculty of Medicine, Chiang Mai University)

  • Jason Gardiner

    (University of California at Los Angeles)

  • Suhua Feng

    (University of California at Los Angeles
    University of California at Los Angeles)

  • Peggy Hsuanyu Kuo

    (University of California at Los Angeles)

  • Steven E. Jacobsen

    (University of California at Los Angeles
    University of California at Los Angeles
    University of California at Los Angeles)

Abstract

The ability to target epigenetic marks like DNA methylation to specific loci is important in both basic research and in crop plant engineering. However, heritability of targeted DNA methylation, how it impacts gene expression, and which epigenetic features are required for proper establishment are mostly unknown. Here, we show that targeting the CG-specific methyltransferase M.SssI with an artificial zinc finger protein can establish heritable CG methylation and silencing of a targeted locus in Arabidopsis. In addition, we observe highly heritable widespread ectopic CG methylation mainly over euchromatic regions. This hypermethylation shows little effect on transcription while it triggers a mild but significant reduction in the accumulation of H2A.Z and H3K27me3. Moreover, ectopic methylation occurs preferentially at less open chromatin that lacks positive histone marks. These results outline general principles of the heritability and interaction of CG methylation with other epigenomic features that should help guide future efforts to engineer epigenomes.

Suggested Citation

  • Wanlu Liu & Javier Gallego-Bartolomé & Yuxing Zhou & Zhenhui Zhong & Ming Wang & Somsakul Pop Wongpalee & Jason Gardiner & Suhua Feng & Peggy Hsuanyu Kuo & Steven E. Jacobsen, 2021. "Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23346-y
    DOI: 10.1038/s41467-021-23346-y
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    Cited by:

    1. Ming Wang & Zhenhui Zhong & Javier Gallego-Bartolomé & Suhua Feng & Yuan-Hsin Shih & Mukun Liu & Jessica Zhou & John Curtis Richey & Charmaine Ng & Yasaman Jami-Alahmadi & James Wohlschlegel & Keqiang, 2023. "Arabidopsis TRB proteins function in H3K4me3 demethylation by recruiting JMJ14," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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