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The protective role of conjunctival goblet cell mucin sialylation

Author

Listed:
  • Moe Matsuzawa

    (Juntendo University Graduate School of Medicine
    Juntendo University Urayasu Hospital
    Juntendo University Graduate School of Medicine)

  • Tomoaki Ando

    (Juntendo University Graduate School of Medicine)

  • Saaya Fukase

    (Juntendo University Graduate School of Medicine
    Juntendo University Urayasu Hospital
    Juntendo University Graduate School of Medicine)

  • Meiko Kimura

    (Juntendo University Graduate School of Medicine
    Juntendo University Urayasu Hospital
    Juntendo University Graduate School of Medicine)

  • Yasuharu Kume

    (Juntendo University Graduate School of Medicine
    Juntendo University Urayasu Hospital
    Juntendo University Graduate School of Medicine)

  • Takuma Ide

    (Juntendo University Graduate School of Medicine
    Juntendo University Graduate School of Medicine)

  • Kumi Izawa

    (Juntendo University Graduate School of Medicine)

  • Ayako Kaitani

    (Juntendo University Graduate School of Medicine)

  • Mutsuko Hara

    (Juntendo University Graduate School of Medicine
    Juntendo University Graduate School of Medicine)

  • Eri Nakamura

    (Juntendo University Graduate School of Medicine)

  • Anna Kamei

    (Juntendo University Graduate School of Medicine
    Juntendo University Graduate School of Medicine)

  • Akira Matsuda

    (Juntendo University Graduate School of Medicine)

  • Nobuhiro Nakano

    (Juntendo University Graduate School of Medicine)

  • Keiko Maeda

    (Juntendo University Graduate School of Medicine
    Juntendo University Graduate School of Medicine)

  • Norihiro Tada

    (Juntendo University Graduate School of Medicine)

  • Hideoki Ogawa

    (Juntendo University Graduate School of Medicine)

  • Ko Okumura

    (Juntendo University Graduate School of Medicine)

  • Akira Murakami

    (Juntendo University Graduate School of Medicine)

  • Nobuyuki Ebihara

    (Juntendo University Urayasu Hospital
    Juntendo University Graduate School of Medicine)

  • Jiro Kitaura

    (Juntendo University Graduate School of Medicine
    Juntendo University Graduate School of Medicine)

Abstract

Gel-forming mucins secreted by conjunctival goblet cells have been implicated in the clearance of allergens, pathogens, and debris. However, their roles remain incompletely understood. Here we show that human and mouse conjunctival goblet cell mucins have Alcian blue-detectable sialic acids, but not sulfates in the steady state. Interestingly, Balb/c mouse strain lacks this sialylation due to a point mutation in a sialyltransferase gene, St6galnac1, which is responsible for sialyl-Tn synthesis. Introduction of intact St6galnac1 to Balb/c restores the sialylation of conjunctival goblet cell mucus. Sialylated mucus efficiently captures and encapsulates the allergen particles in an impenetrable layer, leading to the protection of mice from the development of allergic conjunctivitis. Expression of ST6GALNAC1 and sialyl-Tn is upregulated in humans under conditions with chronic stimuli. These results indicate that the sialylated glycans on the ocular mucins play an essential role in maintaining the conjunctival mucosa by protecting from the incoming foreign bodies such as allergen particles.

Suggested Citation

  • Moe Matsuzawa & Tomoaki Ando & Saaya Fukase & Meiko Kimura & Yasuharu Kume & Takuma Ide & Kumi Izawa & Ayako Kaitani & Mutsuko Hara & Eri Nakamura & Anna Kamei & Akira Matsuda & Nobuhiro Nakano & Keik, 2023. "The protective role of conjunctival goblet cell mucin sialylation," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37101-y
    DOI: 10.1038/s41467-023-37101-y
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    References listed on IDEAS

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    1. Thomas M. Keane & Leo Goodstadt & Petr Danecek & Michael A. White & Kim Wong & Binnaz Yalcin & Andreas Heger & Avigail Agam & Guy Slater & Martin Goodson & Nicholas A. Furlotte & Eleazar Eskin & Chris, 2011. "Mouse genomic variation and its effect on phenotypes and gene regulation," Nature, Nature, vol. 477(7364), pages 289-294, September.
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