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Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification

Author

Listed:
  • Ran Zhang

    (Huazhong Agricultural University
    Hubei University of Technology
    Hubei University of Arts & Science
    University of Georgia)

  • Zhen Hu

    (Huazhong Agricultural University
    Hubei University of Technology
    Hubei University of Arts & Science
    Huazhong Agricultural University)

  • Yanting Wang

    (Huazhong Agricultural University
    Hubei University of Technology
    Hubei University of Arts & Science)

  • Huizhen Hu

    (Huazhong Agricultural University)

  • Fengcheng Li

    (Huazhong Agricultural University)

  • Mi Li

    (University of Tennessee-Knoxville)

  • Arthur Ragauskas

    (University of Tennessee-Knoxville)

  • Tao Xia

    (Huazhong Agricultural University
    Hubei University of Arts & Science
    Huazhong Agricultural University)

  • Heyou Han

    (Huazhong Agricultural University)

  • Jingfeng Tang

    (Hubei University of Technology)

  • Haizhong Yu

    (Hubei University of Arts & Science)

  • Bingqian Xu

    (University of Georgia)

  • Liangcai Peng

    (Huazhong Agricultural University
    Hubei University of Technology
    Hubei University of Arts & Science)

Abstract

Plant cellulose microfibrils are increasingly employed to produce functional nanofibers and nanocrystals for biomaterials, but their catalytic formation and conversion mechanisms remain elusive. Here, we characterize length-reduced cellulose nanofibers assembly in situ accounting for the high density of amorphous cellulose regions in the natural rice fragile culm 16 (Osfc16) mutant defective in cellulose biosynthesis using both classic and advanced atomic force microscopy (AFM) techniques equipped with a single-molecular recognition system. By employing individual types of cellulases, we observe efficient enzymatic catalysis modes in the mutant, due to amorphous and inner-broken cellulose chains elevated as breakpoints for initiating and completing cellulose hydrolyses into higher-yield fermentable sugars. Furthermore, effective chemical catalysis mode is examined in vitro for cellulose nanofibers conversion into nanocrystals with reduced dimensions. Our study addresses how plant cellulose substrates are digestible and convertible, revealing a strategy for precise engineering of cellulose substrates toward cost-effective biofuels and high-quality bioproducts.

Suggested Citation

  • Ran Zhang & Zhen Hu & Yanting Wang & Huizhen Hu & Fengcheng Li & Mi Li & Arthur Ragauskas & Tao Xia & Heyou Han & Jingfeng Tang & Haizhong Yu & Bingqian Xu & Liangcai Peng, 2023. "Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36856-8
    DOI: 10.1038/s41467-023-36856-8
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    References listed on IDEAS

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    1. Dan Ye & Sintu Rongpipi & Sarah N. Kiemle & William J. Barnes & Arielle M. Chaves & Chenhui Zhu & Victoria A. Norman & Alexander Liebman-Peláez & Alexander Hexemer & Michael F. Toney & Alison W. Rober, 2020. "Preferred crystallographic orientation of cellulose in plant primary cell walls," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    2. Wang, Youmei & Liu, Peng & Zhang, Guifen & Yang, Qiaomei & Lu, Jun & Xia, Tao & Peng, Liangcai & Wang, Yanting, 2021. "Cascading of engineered bioenergy plants and fungi sustainable for low-cost bioethanol and high-value biomaterials under green-like biomass processing," Renewable and Sustainable Energy Reviews, Elsevier, vol. 137(C).
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